Differential effects of dimethylsulfoxide on the activities of human DNA polymerases alpha and delta.

Lee MY, Toomey NL
Nucleic acids research (1986), Volume 14, Page 1719
PubMed entry Full article text

Abstract:

The effects of dimethylsulfoxide on the activities of purified human ...
The effects of dimethylsulfoxide on the activities of purified human placental DNA polymerase alpha and DNA polymerase delta were examined. DNA polymerase alpha was inhibited by dimethylsulfoxide, whereas DNA polymerase delta was significantly activated, by as much as 6-fold. Kinetic data show that the effect of dimethylsulfoxide on DNA polymerase delta activity was due to a reduction in the apparent Km for its substrate, dTTP. This novel finding of the differential effects of dimethylsulfoxide on the activities of polymerases alpha and delta may be useful in their identification and differential assay.

Polymerases:

Human Pol delta,Human Pol alpha

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