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Polymerase: 9egN

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Polymerase Reference Property Result Context
9egN Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9 degrees N-7 and mutations affecting 3'-5' exonuclease activity. Molecular Weight 90kD Technique: SDS-PAGE (>95% pure)
9egN Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9 degrees N-7 and mutations affecting 3'-5' exonuclease activity. 3-5' Exonuclease (proofreading) Yes
9egN Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9 degrees N-7 and mutations affecting 3'-5' exonuclease activity. Cloned or native Cloned in E. coli
9egN Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9 degrees N-7 and mutations affecting 3'-5' exonuclease activity. 5-3' Exonuclease No
9egN Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9 degrees N-7 and mutations affecting 3'-5' exonuclease activity. Full length or truncated Full length
9egN Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9 degrees N-7 and mutations affecting 3'-5' exonuclease activity. Specific Activity 2.5E+04 units/mg
9egN Cloning of thermostable DNA polymerases from hyperthermophilic marine Archaea with emphasis on Thermococcus sp. 9 degrees N-7 and mutations affecting 3'-5' exonuclease activity. KM 75uM Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Filter binding ; Experimental conditions: Temp (72°C)
9egN DNA polymerases from hyperthermophiles. 3-5' Exonuclease (proofreading) Yes
9egN DNA polymerases from hyperthermophiles. 3-5' Exo Specific Activity 26 units/mg
9egN DNA polymerases from hyperthermophiles. 5-3' Exonuclease No
9egN DNA polymerases from hyperthermophiles. Overall Error Rate 5.7E-05 errors/bp
9egN DNA polymerases from hyperthermophiles. Specific Activity 0.4 units/mg Technique: Polymerase Assay (calf thymus DNA)
9egN DNA polymerases from hyperthermophiles. Specific Activity 2.5 units/mg Technique: Polymerase Assay (M13 DNA)
9egN DNA polymerases from hyperthermophiles. KM 0.05nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
9egN DNA polymerases from hyperthermophiles. KM 75uM Reaction: Nucleotide incorporation; Substrate: dNTPs
9egN DNA polymerases from hyperthermophiles. Strand Displacement Yes

Version:20241218

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.