Results for:
Polymerase: KF L361A
| Polymerase | Reference | Property | Result | Context |
|---|---|---|---|---|
| KF L361A | The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction. | Specific Activity | 9000 units/mg | Technique: Polymerase assay (poly[d(AT)] |
| KF L361A | The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction. | kcat | 0.044 /second | Reaction: 3-5' Exonuclease; Technique: Steady State (ssDNA, poly(dA)); Experimental conditions: pH (pH 7.5), Mg++ (8mM), Temp (37°C) |
| KF L361A | The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction. | kcat | 4E-05 /second | Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: pH (pH 7.5), Mg++ (6mM), Temp (37°C) |
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