Results for:
Polymerase: KF D424E
Welcome to the results summary page
This page presents all the results for a polymerase, property or reference. To find more information about a polymerase or reference, follow the relevant link.
Using Polbase tables:
Sorting:Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).
Filtering:It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.
Help icons:
The symbol in the upper-right corner of the page
links to this help text. The question mark icon is used everywhere to indicate that help is available.
| Polymerase | Reference | Property | Result | Context |
|---|---|---|---|---|
| KF D424E | The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction. | Specific Activity | 1.1E+04 units/mg | Technique: Polymerase assay (poly[d(AT)] |
| KF D424E | The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction. | kcat | 0.01 /second | Reaction: 3-5' Exonuclease; Technique: Steady State (ssDNA, poly(dA)); Experimental conditions: pH (pH 7.5), Mg++ (8mM), Temp (37°C) |
| KF D424E | The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction. | kcat | 4E-05 /second | Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: pH (pH 7.5), Mg++ (6mM), Temp (37°C) |