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Polymerase: KF D355AE357A

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Polymerase	Reference	Property	Result	Context
KF D355AE357A	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	3-5' Exonuclease (proofreading)	No
KF D355AE357A	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	Frameshift Error Rate	5E-06 errors/bp	Technique: M13mp2 forward mutation assay (1mM dNTP; combined with D424A results)
KF D355AE357A	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	Nucleotide Substitution Rate	2.5E-05 errors/bp	Technique: M13mp2 forward mutation assay (1mM dNTP; combined with D424A results)
KF D355AE357A	Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I.	Other Important Residues	No binding of metals A and B and dNMP
KF D355AE357A	Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I.	Specific Activity	1.11E+04 units/mg	Technique: Polymerase assay (poly[d(AT)]
KF D355AE357A	Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I.	Specific Activity	1.4E-05 units/mg	Technique: Exo activity (arbitrary units) (Relative to WT = 1)