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Polymerase: KF D355AE357A

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Polymerase Reference Property Result Context
KF D355AE357A The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. 3-5' Exonuclease (proofreading) No
KF D355AE357A The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. Frameshift Error Rate 5E-06 errors/bp Technique: M13mp2 forward mutation assay (1mM dNTP; combined with D424A results)
KF D355AE357A The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. Nucleotide Substitution Rate 2.5E-05 errors/bp Technique: M13mp2 forward mutation assay (1mM dNTP; combined with D424A results)
KF D355AE357A Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I. Other Important Residues No binding of metals A and B and dNMP
KF D355AE357A Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I. Specific Activity 1.11E+04 units/mg Technique: Polymerase assay (poly[d(AT)]
KF D355AE357A Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I. Specific Activity 1.4E-05 units/mg Technique: Exo activity (arbitrary units) (Relative to WT = 1)

Using Polbase tables:

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Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.