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Reference: Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases.

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Polymerase Reference Property Result Context
T4 Y254S Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Nucleotide Substitution Rate 36 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
RB69 D222AD327A Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 3-5' Exonuclease (proofreading) No
RB69 beta hairpin- Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 3-5' Exonuclease (proofreading) No
RB69 D222AD327A beta hairpin- Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 5-3' Exonuclease Unspecified
RB69 D222AD327A beta hairpin- Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 3-5' Exonuclease (proofreading) No
T4 G255S Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Nucleotide Substitution Rate 113 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 G255A Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Nucleotide Substitution Rate 107 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Y254Q Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Nucleotide Substitution Rate 47 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Y254K Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Nucleotide Substitution Rate 77 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Y254L Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Nucleotide Substitution Rate 7 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 Y254G Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Nucleotide Substitution Rate 72 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 S256Y Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Nucleotide Substitution Rate 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 S256K Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Nucleotide Substitution Rate 11 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 S256Q Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Nucleotide Substitution Rate 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 S256L Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Nucleotide Substitution Rate 15 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 K257S Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Nucleotide Substitution Rate 1 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 K257Q Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Nucleotide Substitution Rate 4 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 K257Y Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Nucleotide Substitution Rate 3 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 K257L Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Nucleotide Substitution Rate 3 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 K257G Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Nucleotide Substitution Rate 6 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
RB69 G258S Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 3-5' Exonuclease (proofreading) No
RB69 G258SD222A/D237A Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. 3-5' Exonuclease (proofreading) No
T4 S256G Use of 2-aminopurine fluorescence to study the role of the beta hairpin in the proofreading pathway catalyzed by the phage T4 and RB69 DNA polymerases. Nucleotide Substitution Rate 34 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.