Results for:
Reference: Identification of residues critical for the polymerase activity of the Klenow fragment of DNA polymerase I from Escherichia coli., Polymerase: KF Y766S
| Polymerase | Reference | Property | Result | Context |
|---|---|---|---|---|
| KF Y766S | Identification of residues critical for the polymerase activity of the Klenow fragment of DNA polymerase I from Escherichia coli. | kcat | 0.8 /second | Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Rapid quench |
| KF Y766S | Identification of residues critical for the polymerase activity of the Klenow fragment of DNA polymerase I from Escherichia coli. | KM | 6.4uM | Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Rapid quench |
| KF Y766S | Identification of residues critical for the polymerase activity of the Klenow fragment of DNA polymerase I from Escherichia coli. | Kd | 20n/a | Reaction: Polymerase-DNA interaction; Substrate: DNA template; Technique: DNase I Protection ; Experimental conditions: Mg++ (2mM), Temp (22°C), pH (pH 7.5) |
| KF Y766S | Identification of residues critical for the polymerase activity of the Klenow fragment of DNA polymerase I from Escherichia coli. | Cloned or native | Cloned in E. coli |
Showing 1 to 4 of 4 entries