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Reference: Highly efficient incorporation of the fluorescent nucleotide analogs tC and tCO by Klenow fragment.
Polymerase | Reference | Property | Result | Context |
---|---|---|---|---|
Klenow fragment | Highly efficient incorporation of the fluorescent nucleotide analogs tC and tCO by Klenow fragment. | Methods Featured | Fluorescence Spectroscopy | |
Klenow fragment | Highly efficient incorporation of the fluorescent nucleotide analogs tC and tCO by Klenow fragment. | Incorporation of non-standard nucleotides | 95% + | Nucleotide analog: Fluorescent dNTPs |
Klenow fragment | Highly efficient incorporation of the fluorescent nucleotide analogs tC and tCO by Klenow fragment. | Vmax | 8.4 /minute | Reaction: Nucleotide incorporation; Substrate: CTP analog; Technique: Steady State (dtCoTP fluor analogue); Experimental conditions: pH (pH 8.0), Mg++ (10mM), Temp (37°C) |
Klenow fragment | Highly efficient incorporation of the fluorescent nucleotide analogs tC and tCO by Klenow fragment. | Vmax | 5.4 /minute | Reaction: Nucleotide incorporation; Substrate: CTP analog; Technique: Steady State (dtCTP fluor analogue); Experimental conditions: pH (pH 8.0), Mg++ (10mM), Temp (37°C) |
Klenow fragment | Highly efficient incorporation of the fluorescent nucleotide analogs tC and tCO by Klenow fragment. | Vmax | 8.4 /minute | Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Steady State ; Experimental conditions: pH (pH 8.0), Mg++ (10mM), Temp (37°C) |
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