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Reference: The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination.

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Polymerase Reference Property Result Context
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 909uM Reaction: Misincorporation; Substrate: dATP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 761uM Reaction: Misincorporation; Substrate: dGTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 4500uM Reaction: Misincorporation; Substrate: dCTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 725uM Reaction: Misincorporation; Substrate: dGTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 3363uM Reaction: Misincorporation; Substrate: dCTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 1715uM Reaction: Misincorporation; Substrate: dTTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 2085uM Reaction: Misincorporation; Substrate: dCTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 1986uM Reaction: Misincorporation; Substrate: dTTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 1391uM Reaction: Misincorporation; Substrate: dTTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 50uM Reaction: Nucleotide incorporation; Substrate: dATP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 69uM Reaction: Nucleotide incorporation; Substrate: dCTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 26uM Reaction: Nucleotide incorporation; Substrate: dGTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 42uM Reaction: Nucleotide incorporation; Substrate: dTTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 811uM Reaction: Misincorporation; Substrate: dATP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 784uM Reaction: Misincorporation; Substrate: dGTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 1800uM Reaction: Misincorporation; Substrate: dATP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.004 /second Reaction: Misincorporation; Substrate: dATP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.011 /second Reaction: Misincorporation; Substrate: dGTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.77 /second Reaction: Misincorporation; Substrate: dCTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.041 /second Reaction: Misincorporation; Substrate: dGTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.001 /second Reaction: Misincorporation; Substrate: dCTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.09 /second Reaction: Misincorporation; Substrate: dTTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.013 /second Reaction: Misincorporation; Substrate: dCTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.14 /second Reaction: Misincorporation; Substrate: dTTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.015 /second Reaction: Misincorporation; Substrate: dTTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 220 /second Reaction: Nucleotide incorporation; Substrate: dATP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 200 /second Reaction: Nucleotide incorporation; Substrate: dCTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 167 /second Reaction: Nucleotide incorporation; Substrate: dGTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 240 /second Reaction: Nucleotide incorporation; Substrate: dTTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.013 /second Reaction: Misincorporation; Substrate: dATP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.003 /second Reaction: Misincorporation; Substrate: dGTP
RB69 The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.08 /second Reaction: Misincorporation; Substrate: dATP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Frameshift Error Rate 1.2 errors/bp Technique: RB69 rII 131 reversion
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Frameshift Error Rate 4.5 errors/bp Technique: RB69 rII UV232 reversion
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Nucleotide Substitution Rate 2.6 Mutation frequency (relative to WT) Technique: RB69 rII UV256 reversion
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Nucleotide Substitution Rate 4.3 Mutation frequency (relative to WT) Technique: RB69 rII UV375 reversion
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 1467uM Reaction: Misincorporation; Substrate: dCTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 17uM Reaction: Nucleotide incorporation; Substrate: dGTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 28uM Reaction: Misincorporation; Substrate: dTTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 51uM Reaction: Nucleotide incorporation; Substrate: dTTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 205uM Reaction: Misincorporation; Substrate: dTTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 155uM Reaction: Misincorporation; Substrate: dATP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 30uM Reaction: Nucleotide incorporation; Substrate: dATP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 228uM Reaction: Misincorporation; Substrate: dGTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 368uM Reaction: Misincorporation; Substrate: dATP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 310uM Reaction: Misincorporation; Substrate: dATP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 161uM Reaction: Misincorporation; Substrate: dGTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 537uM Reaction: Misincorporation; Substrate: dCTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 251uM Reaction: Misincorporation; Substrate: dGTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 2010uM Reaction: Misincorporation; Substrate: dCTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 50uM Reaction: Nucleotide incorporation; Substrate: dCTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. Kd 507uM Reaction: Misincorporation; Substrate: dTTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.03 /second Reaction: Misincorporation; Substrate: dATP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.11 /second Reaction: Misincorporation; Substrate: dGTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.26 /second Reaction: Misincorporation; Substrate: dCTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.18 /second Reaction: Misincorporation; Substrate: dGTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.03 /second Reaction: Misincorporation; Substrate: dCTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.8 /second Reaction: Misincorporation; Substrate: dTTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.14 /second Reaction: Misincorporation; Substrate: dCTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.05 /second Reaction: Misincorporation; Substrate: dTTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.13 /second Reaction: Misincorporation; Substrate: dTTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 108 /second Reaction: Nucleotide incorporation; Substrate: dATP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 89 /second Reaction: Nucleotide incorporation; Substrate: dGTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 101 /second Reaction: Nucleotide incorporation; Substrate: dCTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 115 /second Reaction: Nucleotide incorporation; Substrate: dTTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.03 /second Reaction: Misincorporation; Substrate: dATP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.01 /second Reaction: Misincorporation; Substrate: dGTP
RB69 L561A The L561A substitution in the nascent base-pair binding pocket of RB69 DNA polymerase reduces base discrimination. kcat 0.75 /second Reaction: Misincorporation; Substrate: dATP

Version:20241007

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Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

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It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.