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Reference: Isolation, characterization, and kinetic properties of truncated forms of T4 DNA polymerase that exhibit 3'-5' exonuclease activity.

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Polymerase Reference Property Result Context
Klenow fragment Isolation, characterization, and kinetic properties of truncated forms of T4 DNA polymerase that exhibit 3'-5' exonuclease activity. KM 3uM Reaction: 3-5' Exonuclease; Substrate: DNA template
Klenow fragment Isolation, characterization, and kinetic properties of truncated forms of T4 DNA polymerase that exhibit 3'-5' exonuclease activity. KM 160uM Reaction: 3-5' Exonuclease; Substrate: DNA template
Klenow fragment Isolation, characterization, and kinetic properties of truncated forms of T4 DNA polymerase that exhibit 3'-5' exonuclease activity. kcat 5.8 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
Klenow fragment Isolation, characterization, and kinetic properties of truncated forms of T4 DNA polymerase that exhibit 3'-5' exonuclease activity. kcat 7.8 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Isolation, characterization, and kinetic properties of truncated forms of T4 DNA polymerase that exhibit 3'-5' exonuclease activity. 3-5' Exonuclease (proofreading) Yes
T4 Isolation, characterization, and kinetic properties of truncated forms of T4 DNA polymerase that exhibit 3'-5' exonuclease activity. KM 1000uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Isolation, characterization, and kinetic properties of truncated forms of T4 DNA polymerase that exhibit 3'-5' exonuclease activity. KM 7uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Isolation, characterization, and kinetic properties of truncated forms of T4 DNA polymerase that exhibit 3'-5' exonuclease activity. kcat 170 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Isolation, characterization, and kinetic properties of truncated forms of T4 DNA polymerase that exhibit 3'-5' exonuclease activity. kcat 420 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 N698 Isolation, characterization, and kinetic properties of truncated forms of T4 DNA polymerase that exhibit 3'-5' exonuclease activity. KM 3300uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 N698 Isolation, characterization, and kinetic properties of truncated forms of T4 DNA polymerase that exhibit 3'-5' exonuclease activity. KM 110uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 N698 Isolation, characterization, and kinetic properties of truncated forms of T4 DNA polymerase that exhibit 3'-5' exonuclease activity. kcat 170 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 N698 Isolation, characterization, and kinetic properties of truncated forms of T4 DNA polymerase that exhibit 3'-5' exonuclease activity. kcat 270 /second Reaction: 3-5' Exonuclease
T4 N388 Isolation, characterization, and kinetic properties of truncated forms of T4 DNA polymerase that exhibit 3'-5' exonuclease activity. KM 3800uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 N388 Isolation, characterization, and kinetic properties of truncated forms of T4 DNA polymerase that exhibit 3'-5' exonuclease activity. KM 1000uM Substrate: DNA template
T4 N388 Isolation, characterization, and kinetic properties of truncated forms of T4 DNA polymerase that exhibit 3'-5' exonuclease activity. kcat 170 /second Reaction: 3-5' Exonuclease; Substrate: DNA template

Version:20240415

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.