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Reference: Kiefer JR1998, Polymerase: Klenow fragment

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Polymerase Reference Property Result Context
Klenow fragment Kiefer JR1998 3-5' Exonuclease (proofreading) Yes
Klenow fragment Kiefer JR1998 kcat 5.2 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Klenow fragment Kiefer JR1998 KM 1.8nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Klenow fragment Kiefer JR1998 KM 2.3uM Reaction: Nucleotide incorporation; Substrate: dNTPs
Klenow fragment Kiefer JR1998 Specific Activity 9700 units/mg Technique: Polymerase Assay (calf thymus DNA)
Klenow fragment Kiefer JR1998 Specific Activity 5500 units/mg Technique: Polymerase Assay (M13 DNA)
Klenow fragment Kiefer JR1998 Processivity 70bp
Klenow fragment Kiefer JR1998 Residues Involved in Catalysis of 3-5' Exo Asp355, Glu357, Asp424, and Asp501
Klenow fragment Kiefer JR1998 Polymerase Catalytic Residue Amino Acids Asp705, Asp882, and Glu883

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.