Results for:
Reference: Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase., Polymerase: Vent A488L
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| Polymerase | Reference | Property | Result | Context |
|---|---|---|---|---|
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | Incorporation of non-standard nucleotides | 10 - 50% | |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | Template lesions | Bypasses | |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | Methods Featured | Gel Shift (EMSA) | |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | Cloned or native | Cloned in E. coli | |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | Tagged | No | |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | Full length or truncated | Unspecified | |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | Application name | Sequencing | |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | Kd | 1100uM | Reaction: Pyrophosphorolysis; Substrate: DNA template; Technique: Rapid quench ; Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C) |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | Kd | 77uM | Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Rapid quench ; Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C) |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | Kd | 360uM | Reaction: Pyrophosphorolysis; Technique: Rapid quench ; Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C) |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | Kd | 18uM | Reaction: Nucleotide incorporation; Substrate: ddCTP; Technique: Rapid quench ; Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C) |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | Kd | 24uM | Reaction: Nucleotide incorporation; Substrate: CTP analog; Technique: Rapid quench ; Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C) |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | kcat | 0.47 /second | Reaction: Nucleotide incorporation; Substrate: ddCTP; Technique: Rapid quench (burst conditions); Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C) |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | kcat | 13 /second | Technique: Rapid quench (single turnover); Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C) |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | kcat | 0.63 /second | Reaction: Pyrophosphorolysis; Substrate: DNA template; Technique: Rapid quench (single turnover); Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C) |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | kcat | 56 /second | Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Rapid quench (single turnover); Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C) |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | kcat | 0.7 /second | Reaction: Nucleotide incorporation; Substrate: rCTP; Technique: Rapid quench (single turnover); Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C) |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | kcat | 0.3 /second | Reaction: Nucleotide incorporation; Substrate: ddCTP; Technique: Rapid quench (single turnover); Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C) |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | kcat | 13 /second | Reaction: Nucleotide incorporation; Substrate: CTP analog; Technique: Rapid quench (single turnover); Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C) |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | kcat | 45 /second | Reaction: Nucleotide incorporation; Substrate: dCTP; Technique: Rapid quench (burst conditions); Experimental conditions: Mg++ (2mM), pH (pH 8.8), Temp (60°C) |
| Vent A488L | Comparative kinetics of nucleotide analog incorporation by vent DNA polymerase. | Gap Filling | Unspecified |