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Reference: The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction., Polymerase: KF D424N, Property: k<sub>cat</sub>
| Polymerase | Reference | Property | Result | Context |
|---|---|---|---|---|
| KF D424N | The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction. | kcat | 2.5E-08 /second | Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: pH (pH 7.5), Mg++ (6mM), Temp (37°C) |
Version:20250305
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