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Reference: Derbyshire V1991, Polymerase: Klenow fragment

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Polymerase Reference Property Result Context
Klenow fragment Derbyshire V1991 Cloned or native Cloned in E. coli
Klenow fragment Derbyshire V1991 Full length or truncated Full length
Klenow fragment Derbyshire V1991 3-5' Exonuclease (proofreading) Yes
Klenow fragment Derbyshire V1991 Tagged No
Klenow fragment Derbyshire V1991 kcat 0.12 /second Reaction: 3-5' Exonuclease; Technique: Steady State (ssDNA: poly(dA)); Experimental conditions: Temp (37°C), Mg++ (8mM), pH (pH 7.5)
Klenow fragment Derbyshire V1991 kcat 0.09 /second Reaction: 3-5' Exonuclease; Technique: Steady State (ssDNA: poly(dT)); Experimental conditions: Temp (37°C), Mg++ (6mM), pH (pH 7.5)
Klenow fragment Derbyshire V1991 KM 0.56uM Reaction: 3-5' Exonuclease; Technique: Steady State (ssDNA: poly(dT)); Experimental conditions: Temp (37°C), Mg++ (6mM), pH (pH 7.5)
Klenow fragment Derbyshire V1991 Specific Activity 9000 units/mg Technique: Polymerase assay (poly[d(AT)]

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.