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Bloom LB

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Publications:

Title Authors Year Journal
A slow ATP-induced conformational change limits the rate of DNA binding but not the rate of beta clamp binding by the escherichia coli gamma complex clamp loader. Bloom LB 2009 The Journal of biological chemistry
Structure/function analysis of the interaction of adenomatous polyposis coli with DNA polymerase beta and its implications for base excision repair. Bloom LB 2007 Biochemistry
A function for the psi subunit in loading the Escherichia coli DNA polymerase sliding clamp. Bloom LB 2007 The Journal of biological chemistry
Multiplexed DNA sequencing-by-synthesis. Bloom LB 2006 Anal Biochem
Dynamics of nucleotide incorporation: snapshots revealed by 2-aminopurine fluorescence studies. Bloom LB 2006 Biochemistry
Dynamics of loading the Escherichia coli DNA polymerase processivity clamp. Bloom LB 2006 Crit Rev Biochem Mol Biol
Increased dNTP binding affinity reveals a nonprocessive role for Escherichia coli beta clamp with DNA polymerase IV. Bloom LB 2004 The Journal of biological chemistry
Mechanism of loading the Escherichia coli DNA polymerase III sliding clamp: I. Two distinct activities for individual ATP sites in the gamma complex. Bloom LB 2004 The Journal of biological chemistry
Mechanism of loading the Escherichia coli DNA polymerase III sliding clamp: II. Uncoupling the beta and DNA binding activities of the gamma complex. Bloom LB 2004 The Journal of biological chemistry
Mechanism of loading the Escherichia coli DNA polymerase III beta sliding clamp on DNA. Bona fide primer/templates preferentially trigger the gamma complex to hydrolyze ATP and load the clamp. Bloom LB 2003 The Journal of biological chemistry
A model for Escherichia coli DNA polymerase III holoenzyme assembly at primer/template ends. DNA triggers a change in binding specificity of the gamma complex clamp loader. Bloom LB 2000 The Journal of biological chemistry
Molecular mechanism and energetics of clamp assembly in Escherichia coli. The role of ATP hydrolysis when gamma complex loads beta on DNA. Bloom LB 2000 The Journal of biological chemistry
Division of labor--sequential ATP hydrolysis drives assembly of a DNA polymerase sliding clamp around DNA. Bloom LB 1999 The EMBO journal
Exonuclease-polymerase active site partitioning of primer-template DNA strands and equilibrium Mg2+ binding properties of bacteriophage T4 DNA polymerase. Bloom LB 1998 Biochemistry
Stopped-flow fluorescence study of precatalytic primer strand base-unstacking transitions in the exonuclease cleft of bacteriophage T4 DNA polymerase. Bloom LB 1998 Biochemistry
The proofreading pathway of bacteriophage T4 DNA polymerase. Bloom LB 1998 The Journal of biological chemistry
Pre-steady state analysis of the assembly of wild type and mutant circular clamps of Escherichia coli DNA polymerase III onto DNA. Bloom LB 1998 The Journal of biological chemistry
Fidelity of Escherichia coli DNA polymerase III holoenzyme. The effects of beta, gamma complex processivity proteins and epsilon proofreading exonuclease on nucleotide misincorporation efficiencies. Bloom LB 1997 The Journal of biological chemistry
Dynamics of loading the beta sliding clamp of DNA polymerase III onto DNA. Bloom LB 1996 The Journal of biological chemistry
Nucleotide insertion and primer extension at abasic template sites in different sequence contexts. Bloom LB 1994 Annals of the New York Academy of Sciences
Pre-steady-state kinetic analysis of sequence-dependent nucleotide excision by the 3'-exonuclease activity of bacteriophage T4 DNA polymerase. Bloom LB 1994 Biochemistry
Effect of a template-located 2',2'-difluorodeoxycytidine on the kinetics and fidelity of base insertion by Klenow (3'-->5'exonuclease-) fragment. Bloom LB 1993 Cancer research
Ionization of bromouracil and fluorouracil stimulates base mispairing frequencies with guanine. Bloom LB 1993 The Journal of biological chemistry
Kinetics of deoxyribonucleotide insertion and extension at abasic template lesions in different sequence contexts using HIV-1 reverse transcriptase. Bloom LB 1993 The Journal of biological chemistry
Biochemical basis of DNA replication fidelity. Bloom LB 1993 Crit Rev Biochem Mol Biol
Influence of 5'-nearest neighbors on the insertion kinetics of the fluorescent nucleotide analog 2-aminopurine by Klenow fragment. Bloom LB 1993 Biochemistry
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