Dynamics of nucleotide incorporation: snapshots revealed by 2-aminopurine fluorescence studies.

Abstract:

Formation of a noncanonical base pair between dFTP, a dTTP analogue ...

Polymerases:

Topics:

Biotech Applications, Kinetic Parameters, Nucleotide Analogs / Template Lesions, Fidelity, Nucleotide Incorporation, Methods

One line summary:

Highly accurate DNA polymerases, such as the T4 DNA polymerase, achieve high fidelity in nucleotide selection by using multiple binding steps with each successive step having increased stringency.

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 D112AE114A Dynamics of nucleotide incorporation: snapshots revealed by 2-aminopurine fluorescence studies. Kd 31uM Reaction: Nucleotide incorporation; Substrate: dTTP; Technique: Rapid quench (across template 2AP)

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