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Welcome to the Polymerase Page

This page presents all the information in Polbase for Sequenase.


At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.


If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.

External links:

Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.


All references that refer to this Polymerase are listed with their topics and paper's title in the References table.


All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.

Help icons:

The show help symbol in the upper-right corner of the page links to this help text. The question mark icon is used everywhere to indicate that help is available.

Mutant of: T7 ()

Sequenase is a family A enzyme from bacteriophage T7.

Selected Properties for Sequenase:

3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
No (1) no data no data 3.4e-05-4.4e-05 errors/bp (2) 4.4e-05-5.4e-05 errors/bp (2)
Year Authors Title Results by property
2008 Linda J Reha-Krantz Reha-Krantz LJ2008
1998 P Keohavong, R Shukla, A Melacrinos, B W Day, Linda J Reha-Krantz Keohavong P1998
1992 P A Garrity, B J Wold Garrity PA1992
1991 N F Cariello, James A Swenberg, T R Skopek Cariello NF1991 3-5' exo,Nucleotide Substitution Rate,Application name,All properties
1991 L L Ling, P Keohavong, C Dias, William G Thilly Ling LL1991 Overall Error Rate,All properties
1989 P Keohavong, William G Thilly Keohavong P1989 Nucleotide Substitution Rate,All properties
All Polymerases | 6 Results

Using Polbase tables:


Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).


It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.