Promega GoTaq G2 Hot Start

From Unknown (limited name: Promega GoTaq G2 Hot Start)
show help

Welcome to the Polymerase Page

This page presents all the information in Polbase for Promega GoTaq G2 Hot Start.

Mutants:

At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.

Structures/Sequence:

If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.

External links:

Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.

References:

All references that refer to this Polymerase are listed with their topics and paper's title in the References table.

Results:

All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.

Help icons:

The show help symbol in the upper-right corner of the page links to this help text. The question mark icon is used everywhere to indicate that help is available.

Promega GoTaq G2 Hot Start is a family A enzyme from Unknown.

Commercially available from Promega of catalog number M7401. 1.

Recommended raction setup:

Combine the following components on ice:

Component                                                      Final Volume                            Final Concentration

5X Green or Colorless GoTaq® Flexi Buffer1             10µl                                           1X

MgCl2 Solution, 25mM                                           2–8µl                                       1.0–4.0mM

PCR Nucleotide Mix, 10mM each                               1µl                                    0.2mM each dNTP

upstream primer                                                    Xµl                                         0.1–1.0µM

downstream primer                                                Yµl                                         0.1–1.0µM

GoTaq® G2 Hot Start Polymerase (5u/µl)              0.25µl                                        1.25u

template DNA                                                       Zµl                                         <0.5µg/50µl

Nuclease-Free Water to 50µl

 

Recommended cycling conditions:

  1. initial 2 minute denaturation at 94oC
  2. following denaturation steps for 15 seconds - 1 minute
  3. Annealing 15 seconds - 1 minute
  4. Extension of 1 minute per kb at 72oC with a final extension of 5 minutes

Selected Properties for Promega GoTaq G2 Hot Start:

 3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
no data no data no data no data no data

Version:20240415

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.