Promega GoTaq G2 Hot Start

From Unknown (limited name: Promega GoTaq G2 Hot Start)
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This page presents all the information in Polbase for Promega GoTaq G2 Hot Start.

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Promega GoTaq G2 Hot Start is a family A enzyme from Unknown.

Commercially available from Promega of catalog number M7401. 1.

Recommended raction setup:

Combine the following components on ice:

Component                                                      Final Volume                            Final Concentration

5X Green or Colorless GoTaq® Flexi Buffer1             10µl                                           1X

MgCl2 Solution, 25mM                                           2–8µl                                       1.0–4.0mM

PCR Nucleotide Mix, 10mM each                               1µl                                    0.2mM each dNTP

upstream primer                                                    Xµl                                         0.1–1.0µM

downstream primer                                                Yµl                                         0.1–1.0µM

GoTaq® G2 Hot Start Polymerase (5u/µl)              0.25µl                                        1.25u

template DNA                                                       Zµl                                         <0.5µg/50µl

Nuclease-Free Water to 50µl

 

Recommended cycling conditions:

  1. initial 2 minute denaturation at 94oC
  2. following denaturation steps for 15 seconds - 1 minute
  3. Annealing 15 seconds - 1 minute
  4. Extension of 1 minute per kb at 72oC with a final extension of 5 minutes

Selected Properties for Promega GoTaq G2 Hot Start:

 3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
no data no data no data no data no data

Version:20241218

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