Thermofisher DreamTaq Hot Start DNA Polymerase

From Unknown (limited name: Thermofisher DreamTaq Hot Start)
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This page presents all the information in Polbase for Thermofisher DreamTaq Hot Start DNA Polymerase.

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Thermofisher DreamTaq Hot Start DNA Polymerase is a family A enzyme from Unknown.

DreamTaq Hot Start DNA polymerase is available from Thermo-Fisher. The catalog number is EP1701.

PCR setup is as follows:

10X DreamTaq Buffer*: 5 µL, dNTP Mix 2 mM each (#R0241): 5 µL (0.2 mM of each), Forward primer: 0.1–1.0 µM, Reverse primer 0.1–1.0 µM, Template DNA 10 pg–1 µg, DreamTaq Hot Start DNA Polymerase: 1.25 U, Water to 50 µL.

 

Cycling condtitions are as follows:

Initial denaturation 95C  1–3 min

Denaturation 95C 30s, Annealing Tm 30 s, 25–40 Extension 72C 1 min:  for 25-40 cycles

Final Extension 72C 5–15 min

Selected Properties for Thermofisher DreamTaq Hot Start DNA Polymerase:

3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
no data no data no data no data no data
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