Thermofisher DreamTaq Hot Start DNA Polymerase
From
Unknown
(limited name: Thermofisher DreamTaq Hot Start)
Thermofisher DreamTaq Hot Start DNA Polymerase
is a family A enzyme
from
Unknown.
DreamTaq Hot Start DNA polymerase is available from Thermo-Fisher. The catalog number is EP1701.
PCR setup is as follows:
10X DreamTaq Buffer*: 5 µL, dNTP Mix 2 mM each (#R0241): 5 µL (0.2 mM of each), Forward primer: 0.1–1.0 µM, Reverse primer 0.1–1.0 µM, Template DNA 10 pg–1 µg, DreamTaq Hot Start DNA Polymerase: 1.25 U, Water to 50 µL.
Cycling condtitions are as follows:
Initial denaturation 95C 1–3 min
Denaturation 95C 30s, Annealing Tm 30 s, 25–40 Extension 72C 1 min: for 25-40 cycles
Final Extension 72C 5–15 min
Selected Properties for Thermofisher DreamTaq Hot Start DNA Polymerase: |
3'-5' exo activity | 5'-3' exo activity | Frameshift Rate | Substitution Rate | General Error Rate |
|---|---|---|---|---|---|
| no data | no data | no data | no data | no data |
- References (0)
- Structures (0)
- Sequences (0)
- Mutants (0)...
- Formulations (0)
- Polymerase Lots (0)
- External links
| Year | Authors | Title | Results by property |
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Protein information: search for Thermofisher DreamTaq Hot Start DNA Polymerase at UniProt
Sequence information: NCBI nucleotides European nucleotide archive