Qiagen HotStarTaq Plus
HotStarTaq Plus DNA polymerase is available from Qiagen with the product ID 203601. It is a hot start polymerase that works using a chemical modification. Product details can be found at the link: https://www.qiagen.com/us/products/discovery-and-translational-research/pcr-qpcr/pcr-enzymes-and-kits/end-point-pcr/hotstartaq-plus-dna-polymerase/?clear=true#orderinginformation
The recommeded reaction setup is as follows:
Component Volume/Reaction Final Concentration
10X PCR Buffer of CoralLoad PCR Buffer 10ul 1X
dNTP Mix (10mM each) 2ul 200uM each dNTP
Primers variable 0.1-0.5 uM each
HotStarTaq Plus DNA Polymerase 0.5ul 2.5 units/raection
Optional: 5X Q-solution 20ul 1X
Template DNA variable < 1ug/reaction
Total reaction volume: 100ul
Cycling conditions are recommended as follows:
95oC 5 minutes initial denaturation
30 cycles of:
94oC 30 seconds
Tm-5oC 30 seconds
72oC 1 minute
72oC 10 minutes final extension
Selected Properties for Qiagen HotStarTaq Plus: |
3'-5' exo activity | 5'-3' exo activity | Frameshift Rate | Substitution Rate | General Error Rate |
|---|---|---|---|---|---|
| no data | no data | no data | no data | no data |
- References (0)
- Structures (0)
- Sequences (0)
- Mutants (0)...
- Formulations (0)
- Polymerase Lots (0)
- External links
| Year | Authors | Title | Results by property |
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Protein information: search for Qiagen HotStarTaq Plus at UniProt
Sequence information: NCBI nucleotides European nucleotide archive