Qiagen HotStarTaq Plus

From Unknown (limited name: Qiagen HotStarTaq Plus)
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Welcome to the Polymerase Page

This page presents all the information in Polbase for Qiagen HotStarTaq Plus.

Mutants:

At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.

Structures/Sequence:

If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.

External links:

Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.

References:

All references that refer to this Polymerase are listed with their topics and paper's title in the References table.

Results:

All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.

Help icons:

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Qiagen HotStarTaq Plus is a family A enzyme from Unknown.

HotStarTaq Plus DNA polymerase is available from Qiagen with the product ID 203601. It is a hot start polymerase that works using a chemical modification. Product details can be found at the link: https://www.qiagen.com/us/products/discovery-and-translational-research/pcr-qpcr/pcr-enzymes-and-kits/end-point-pcr/hotstartaq-plus-dna-polymerase/?clear=true#orderinginformation

The recommeded reaction setup is as follows:

Component                                                                    Volume/Reaction                            Final Concentration

10X PCR Buffer of CoralLoad PCR Buffer                                   10ul                                                     1X

dNTP Mix (10mM each)                                                             2ul                                           200uM each dNTP

Primers                                                                               variable                                         0.1-0.5 uM each

HotStarTaq Plus DNA Polymerase                                            0.5ul                                          2.5 units/raection

Optional: 5X Q-solution                                                           20ul                                                    1X

Template DNA                                                                     variable                                        < 1ug/reaction

 

Total reaction volume: 100ul

 

 

Cycling conditions are recommended as follows:

       95oC    5 minutes                   initial denaturation

       30 cycles of:

             94oC        30 seconds

             Tm-5oC    30 seconds

             72oC        1 minute

       72oC     10 minutes                 final extension        

Selected Properties for Qiagen HotStarTaq Plus:

 3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
no data no data no data no data no data

Version:20240415

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