Qiagen HotStarTaq Plus

From Unknown (limited name: Qiagen HotStarTaq Plus)
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Welcome to the Polymerase Page

This page presents all the information in Polbase for Qiagen HotStarTaq Plus.


At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.


If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.

External links:

Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.


All references that refer to this Polymerase are listed with their topics and paper's title in the References table.


All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.

Help icons:

The show help symbol in the upper-right corner of the page links to this help text. The question mark icon is used everywhere to indicate that help is available.

Qiagen HotStarTaq Plus is a family A enzyme from Unknown.

HotStarTaq Plus DNA polymerase is available from Qiagen with the product ID 203601. It is a hot start polymerase that works using a chemical modification. Product details can be found at the link: https://www.qiagen.com/us/products/discovery-and-translational-research/pcr-qpcr/pcr-enzymes-and-kits/end-point-pcr/hotstartaq-plus-dna-polymerase/?clear=true#orderinginformation

The recommeded reaction setup is as follows:

Component                                                                    Volume/Reaction                            Final Concentration

10X PCR Buffer of CoralLoad PCR Buffer                                   10ul                                                     1X

dNTP Mix (10mM each)                                                             2ul                                           200uM each dNTP

Primers                                                                               variable                                         0.1-0.5 uM each

HotStarTaq Plus DNA Polymerase                                            0.5ul                                          2.5 units/raection

Optional: 5X Q-solution                                                           20ul                                                    1X

Template DNA                                                                     variable                                        < 1ug/reaction


Total reaction volume: 100ul



Cycling conditions are recommended as follows:

       95oC    5 minutes                   initial denaturation

       30 cycles of:

             94oC        30 seconds

             Tm-5oC    30 seconds

             72oC        1 minute

       72oC     10 minutes                 final extension        

Selected Properties for Qiagen HotStarTaq Plus:

3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
no data no data no data no data no data

Using Polbase tables:


Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).


It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.