BioRad iTaq DNA polymerase

From Unknown (limited name: BioRad iTaq DNA polymerase)
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Welcome to the Polymerase Page

This page presents all the information in Polbase for BioRad iTaq DNA polymerase.


At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.


If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.

External links:

Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.


All references that refer to this Polymerase are listed with their topics and paper's title in the References table.


All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.

Help icons:

The show help symbol in the upper-right corner of the page links to this help text. The question mark icon is used everywhere to indicate that help is available.

BioRad iTaq DNA polymerase is a family A enzyme from Unknown.

iTaq is a Hot Start DNA polymerase available from BioRad with the catalog number 1708870.


PCR setup is as follows:

Component                     Volume per 50ul reaction                      Final Concentration

10X iTaq buffer                              5.0ul                                                      1X

50mM MgCl2                                  1.5ul                                                   1.5mM

10mM dNTP                                   1.0ul                                                200uM each

iTaq DNA polymerase                    0.25ul                                                    1.25U

Primers                                                                                            100nM-500nM each

DNA template

Water                                         to 50ul


Recommended Cycling Conditions are as follows:

Polymerase activation:          95oC, 3min

25-40 cycles of:                   95oC, 30sec

                                          55oC, 30sec

                                          72oC, 1min

Hold at 4oC

Selected Properties for BioRad iTaq DNA polymerase:

3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
no data no data no data no data no data

Using Polbase tables:


Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).


It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.