Invitrogen Platinum II Taq Hot Start DNA polymerase

From Unknown (limited name: Invitrogen Platinum II Taq Hot Start DNA polymerase)
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This page presents all the information in Polbase for Invitrogen Platinum II Taq Hot Start DNA polymerase.

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Invitrogen Platinum II Taq Hot Start DNA polymerase is a family A enzyme from Unknown.

Platinum II Taq Hot Start DNA polymerase is available from Thermofisher with the catalog number 14966001.

 

Recommended PCR setup:

Component                                       Volume per 50ul reaction                 Final Concentration

5X Platinum II PCR Buffer                                   10ul                                                1X

10nM dNTP                                                         1ul                                         0.2mM each

Platinum II Taq Hot Start Polymerase                 0.4ul                                           0.04 U/ul

Platinum GC enhancer (optional)*                       10ul                                                1X

nuclease-free water to 50ul

 

*GC enhancer is recommended with targets with >65% GC sequences

 

 

Recommened Cycling Conditions:

initial denaturation              94oC, 2min

25-35 cycles of:                 94oC, 15sec

                                        60oC, 15sec*

                                        68oC, 15sec/kb*

4oC hold

 

*Steps can be combined to be 60oC for 15 seconds

Selected Properties for Invitrogen Platinum II Taq Hot Start DNA polymerase:

 3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
no data no data no data no data no data

Version:20240415

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