Invitrogen Platinum II Taq Hot Start DNA polymerase
Platinum II Taq Hot Start DNA polymerase is available from Thermofisher with the catalog number 14966001.
Recommended PCR setup:
Component Volume per 50ul reaction Final Concentration
5X Platinum II PCR Buffer 10ul 1X
10nM dNTP 1ul 0.2mM each
Platinum II Taq Hot Start Polymerase 0.4ul 0.04 U/ul
Platinum GC enhancer (optional)* 10ul 1X
nuclease-free water to 50ul
*GC enhancer is recommended with targets with >65% GC sequences
Recommened Cycling Conditions:
initial denaturation 94oC, 2min
25-35 cycles of: 94oC, 15sec
60oC, 15sec*
68oC, 15sec/kb*
4oC hold
*Steps can be combined to be 60oC for 15 seconds
Selected Properties for Invitrogen Platinum II Taq Hot Start DNA polymerase: |
3'-5' exo activity | 5'-3' exo activity | Frameshift Rate | Substitution Rate | General Error Rate |
---|---|---|---|---|---|
no data | no data | no data | no data | no data |
- References (0)
- Structures (0)
- Sequences (0)
- Mutants (0)...
- Formulations (0)
- Polymerase Lots (0)
- External links
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Protein information: search for Invitrogen Platinum II Taq Hot Start DNA polymerase at UniProt
Sequence information: NCBI nucleotides European nucleotide archive