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Polymerase: T4 D324A

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Polymerase Reference Property Result Context
T4 D324A Genetic and biochemical studies of bacteriophage T4 DNA polymerase 3'-->5'-exonuclease activity. Nucleotide Substitution Rate 60 Mutation frequency (relative to WT) Technique: T4 rII 199oc reversion assay
T4 D324A Mutational and pH studies of the 3' --> 5' exonuclease activity of bacteriophage T4 DNA polymerase. kcat 0.01 /second Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Degradation of labeled single-stranded DNA (9.5)
T4 D324A Kinetics of error generation in homologous B-family DNA polymerases. Kd 18uM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T)
T4 D324A Kinetics of error generation in homologous B-family DNA polymerases. Kd 897uM Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F)
T4 D324A Kinetics of error generation in homologous B-family DNA polymerases. kcat 180 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T)
T4 D324A Kinetics of error generation in homologous B-family DNA polymerases. kcat 2.13 /second Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F)
T4 D324A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. KM 6.3uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D324A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. KM 0.4uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D324A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. kcat 0.0062 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D324A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. kcat 7E-05 /second Reaction: 3-5' Exonuclease; Substrate: DNA template

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.