Results for:
Polymerase: T4 D324A
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| Polymerase | Reference | Property | Result | Context |
|---|---|---|---|---|
| T4 D324A | Mutational and pH studies of the 3' --> 5' exonuclease activity of bacteriophage T4 DNA polymerase. | kcat | 0.01 /second | Reaction: 3-5' Exonuclease; Substrate: DNA template; Technique: Degradation of labeled single-stranded DNA (9.5) |
| T4 D324A | Kinetics of error generation in homologous B-family DNA polymerases. | Kd | 18uM | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T) |
| T4 D324A | Kinetics of error generation in homologous B-family DNA polymerases. | Kd | 897uM | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F) |
| T4 D324A | Kinetics of error generation in homologous B-family DNA polymerases. | kcat | 180 /second | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template T) |
| T4 D324A | Kinetics of error generation in homologous B-family DNA polymerases. | kcat | 2.13 /second | Reaction: Nucleotide incorporation; Substrate: dATP; Technique: Single Turnover (across template F) |
| T4 D324A | Genetic and biochemical studies of bacteriophage T4 DNA polymerase 3'-->5'-exonuclease activity. | Nucleotide Substitution Rate | 60 Mutation frequency (relative to WT) | Technique: T4 rII 199oc reversion assay |
| T4 D324A | Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. | KM | 6.3uM | Reaction: 3-5' Exonuclease; Substrate: DNA template |
| T4 D324A | Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. | KM | 0.4uM | Reaction: 3-5' Exonuclease; Substrate: DNA template |
| T4 D324A | Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. | kcat | 0.0062 /second | Reaction: 3-5' Exonuclease; Substrate: DNA template |
| T4 D324A | Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. | kcat | 7E-05 /second | Reaction: 3-5' Exonuclease; Substrate: DNA template |