Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase.

Abstract:

Three groups of T4 DNA polymerase mutants were prepared and ...

Polymerases:

Topics:

Mutational Analysis, Modulators/Inhibitors, Kinetic Parameters, Structure and Structure/Function, Nucleotide Incorporation, Exonuclease Activity, Enzyme Substrate Interactions

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
T4 Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. KM 6.9uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. KM 0.4uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. kcat 65.1 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. kcat 0.16 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D112A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. KM 7.7uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D112A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. KM 1.6uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D112A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. kcat 0.0078 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D112A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. kcat 0.0037 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D112N Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. KM 4uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D112N Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. kcat 0.001 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 E114A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. KM 5uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 E114A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. KM 2uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 E114A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. kcat 2 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 E114A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. kcat 0.08 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219AD324A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. KM 6.7uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219AD324A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. kcat 0.0034 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. KM 12.8uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. KM 0.3uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. kcat 0.096 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D219A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. kcat 6E-05 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D324A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. KM 6.3uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D324A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. KM 0.4uM Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D324A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. kcat 0.0062 /second Reaction: 3-5' Exonuclease; Substrate: DNA template
T4 D324A Functional consequences and exonuclease kinetic parameters of point mutations in bacteriophage T4 DNA polymerase. kcat 7E-05 /second Reaction: 3-5' Exonuclease; Substrate: DNA template

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