Learning about DNA polymerase function by studying antimutator DNA polymerases.


Mutant bacteriophage T4 DNA polymerases exist that appear primarily to reduce the frequency of AT-to-GC transitions when this [antimutator' phenotype is assessed by genetic methods. This observation disagrees with in vitro studies, which indicate that T4 antimutator DNA polymerases have increased proofreading abilities and effectively edit all types of base substitution errors. One explanation that reconciles the apparent in vivo mutational specificity of antimutator DNA polymerases with their biochemical properties is that the in vivo mutational specificity identifies mismatched primer-termini that are corrected less efficiently by the wild-type level of proofreading activity, but are corrected if proofreading is increased.



Mutational Analysis, Fidelity, Nucleotide Incorporation, Exonuclease Activity


new topics/pols set partial results complete validated

Entry validated by:

Log in to edit reference All References

Using Polbase tables:


Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).


It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.