Efficient enzymatic synthesis of LNA-modified DNA duplexes using KOD DNA polymerase.


Three different LNA-nucleoside triphosphates, LNA-TTP, LNA-ATP and ...
Three different LNA-nucleoside triphosphates, LNA-TTP, LNA-ATP and LNA-5-methyl-CTP, were investigated as substrates for KOD DNA polymerase. The results reveal that KOD DNA polymerase is an efficient catalyst for template directed synthesis of DNA oligonucleotide duplexes containing a large number of LNA nucleotides by primer extension reactions. Furthermore, KOD DNA polymerase is shown to be suitable for the PCR amplification of an LNA-modified DNA duplex.



Nucleotide Analogs / Template Lesions


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