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Reference: Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.

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Polymerase	Reference	Property	Result	Context
Sce Pol alpha	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Molecular Weight	180kD
Sce Pol alpha	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	3-5' Exonuclease (proofreading)	No
Sce Pol alpha	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	5-3' Exonuclease	No
Sce Pol alpha	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Processivity	10bp
Sce Pol alpha	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Specific Activity	90.2 units/mg	Technique: Polymerase assay (poly[d(AT)]
Sce Pol alpha	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Specific Activity	219 units/mg	Technique: Polymerase Assay (calf thymus DNA)
Sce Pol alpha	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	K_M	63nM	Reaction: Polymerase-DNA interaction; Substrate: DNA template
Sce Pol alpha	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	K_M	3.8uM	Reaction: Nucleotide incorporation; Substrate: dNTPs
Sce Pol II	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Molecular Weight	145kD
Sce Pol II	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	3-5' Exonuclease (proofreading)	Yes
Sce Pol II	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Full length or truncated	Full length
Sce Pol II	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Processivity	100bp
Sce Pol II	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Specific Activity	87.4 units/mg	Technique: Polymerase assay (poly[d(AT)]
Sce Pol II	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Specific Activity	176 units/mg	Technique: Polymerase Assay (calf thymus DNA)
Sce Pol II	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	K_M	6.2uM	Reaction: Nucleotide incorporation; Substrate: dNTPs
Sce Pol II*	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Molecular Weight	200kD
Sce Pol II*	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	3-5' Exonuclease (proofreading)	Yes
Sce Pol II*	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	5-3' Exonuclease	No
Sce Pol II*	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Processivity	100bp
Sce Pol II*	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Specific Activity	243 units/mg	Technique: Polymerase Assay (calf thymus DNA)
Sce Pol II*	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Specific Activity	333 units/mg	Technique: Polymerase assay (poly[d(AT)]
Sce Pol II*	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	K_M	7.5uM	Reaction: Nucleotide incorporation; Substrate: dNTPs
Sce Pol II*	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	K_M	26nM	Reaction: Polymerase-DNA interaction; Substrate: DNA template
Sce Pol III	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Molecular Weight	125kD
Sce Pol III	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	3-5' Exonuclease (proofreading)	Yes
Sce Pol III	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Specific Activity	45 units/mg	Technique: Polymerase Assay (calf thymus DNA)
Sce Pol III	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Specific Activity	3.3 units/mg	Technique: Polymerase assay (poly[d(AT)]