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Reference: The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.

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Polymerase	Reference	Property	Result	Context
KF D424A	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	3-5' Exonuclease (proofreading)	No
KF D424A	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	Frameshift Error Rate	0.0043 errors/bp	Technique: Forward mutational (1 uM dNTP)
KF D424A	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	Nucleotide Substitution Rate	0.00012 errors/bp	Technique: Reversion (1 uM dNTP)
Klenow fragment	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	3-5' Exonuclease (proofreading)	Yes
Klenow fragment	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	Residues Involved in Catalysis of 3-5' Exo	D424, D355, E357, D501
Klenow fragment	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	Frameshift Error Rate	6E-07 errors/bp	Technique: M13mp2 forward mutation assay (1 uM dNTP, error rate is upper limit (≤))
Klenow fragment	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	Nucleotide Substitution Rate	5.8E-06 errors/bp	Technique: M13mp2 forward mutation assay (1 uM dNTP)
KF D424A	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	3-5' Exonuclease (proofreading)	No
KF D424A	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	Frameshift Error Rate	5E-06 errors/bp	Technique: M13mp2 forward mutation assay (1mM dNTP; combined with D355A,E357A results)
KF D424A	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	Nucleotide Substitution Rate	2.5E-05 errors/bp	Technique: M13mp2 forward mutation assay (1mM dNTP; combined with D355A,E357A results)
KF D355AE357A	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	3-5' Exonuclease (proofreading)	No
KF D355AE357A	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	Frameshift Error Rate	5E-06 errors/bp	Technique: M13mp2 forward mutation assay (1mM dNTP; combined with D424A results)
KF D355AE357A	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	Nucleotide Substitution Rate	2.5E-05 errors/bp	Technique: M13mp2 forward mutation assay (1mM dNTP; combined with D424A results)
KF(515-928)	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	3-5' Exonuclease (proofreading)	No
KF(515-928)	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	Frameshift Error Rate	1.3E-05 errors/bp	Technique: M13mp2 forward mutation assay (1mM dNTP)
KF(515-928)	The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.	Nucleotide Substitution Rate	3.2E-05 errors/bp	Technique: M13mp2 forward mutation assay (1mM dNTP)