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Reference: The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I.

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Polymerase Reference Property Result Context
Klenow fragment The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. 3-5' Exonuclease (proofreading) Yes
Klenow fragment The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. Residues Involved in Catalysis of 3-5' Exo D424, D355, E357, D501
Klenow fragment The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. Frameshift Error Rate 6E-07 errors/bp Technique: M13mp2 forward mutation assay (1 uM dNTP, error rate is upper limit (≤))
Klenow fragment The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. Nucleotide Substitution Rate 5.8E-06 errors/bp Technique: M13mp2 forward mutation assay (1 uM dNTP)
KF D424A The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. 3-5' Exonuclease (proofreading) No
KF D424A The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. Frameshift Error Rate 5E-06 errors/bp Technique: M13mp2 forward mutation assay (1mM dNTP; combined with D355A,E357A results)
KF D424A The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. Nucleotide Substitution Rate 2.5E-05 errors/bp Technique: M13mp2 forward mutation assay (1mM dNTP; combined with D355A,E357A results)
KF D355AE357A The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. 3-5' Exonuclease (proofreading) No
KF D355AE357A The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. Frameshift Error Rate 5E-06 errors/bp Technique: M13mp2 forward mutation assay (1mM dNTP; combined with D424A results)
KF D355AE357A The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. Nucleotide Substitution Rate 2.5E-05 errors/bp Technique: M13mp2 forward mutation assay (1mM dNTP; combined with D424A results)
KF(515-928) The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. 3-5' Exonuclease (proofreading) No
KF(515-928) The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. Frameshift Error Rate 1.3E-05 errors/bp Technique: M13mp2 forward mutation assay (1mM dNTP)
KF(515-928) The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. Nucleotide Substitution Rate 3.2E-05 errors/bp Technique: M13mp2 forward mutation assay (1mM dNTP)
KF D424A The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. 3-5' Exonuclease (proofreading) No
KF D424A The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. Frameshift Error Rate 0.0043 errors/bp Technique: Forward mutational (1 uM dNTP)
KF D424A The fidelity of DNA synthesis catalyzed by derivatives of Escherichia coli DNA polymerase I. Nucleotide Substitution Rate 0.00012 errors/bp Technique: Reversion (1 uM dNTP)

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.