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Reference: A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity.

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Polymerase	Reference	Property	Result	Context
Klenow fragment	A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity.	Molecular Weight	6.8E+04 Dalton
Klenow fragment	A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity.	Cloned or native	Native organism
Klenow fragment	A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity.	Tagged	No
Klenow fragment	A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity.	Frameshift Error Rate	0.0102 errors/bp	Technique: Reversion
Klenow fragment	A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity.	Overall Error Rate	4E-05 errors/bp
Klenow fragment	A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity.	Nucleotide Substitution Rate	0.00036 errors/bp	Technique: Reversion
Klenow fragment	A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity.	Nucleotide Substitution Rate	0.0028 errors/bp	Technique: Forward mutational
Klenow fragment	A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity.	Full length or truncated	Full length
Klenow fragment	A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity.	K_M	3.7uM	Reaction: Nucleotide incorporation; Substrate: dTTP
Klenow fragment	A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity.	K_d	42nM	Reaction: Polymerase-DNA interaction; Substrate: DNA template
Klenow fragment	A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity.	k_cat	0.3 /second	Reaction: Nucleotide incorporation; Substrate: dTTP

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