A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity.

Abstract:

In Klenow fragment DNA polymerase, a flexible 50-amino acid subdomain ...

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Kinetic Parameters, Fidelity, Source / Purification

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Klenow fragment A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity. Molecular Weight 6.8E+04 Dalton
Klenow fragment A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity. Cloned or native Native organism
Klenow fragment A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity. Tagged No
Klenow fragment A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity. Frameshift Error Rate 0.0102 errors/bp Technique: Reversion
Klenow fragment A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity. Overall Error Rate 4E-05 errors/bp
Klenow fragment A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity. Nucleotide Substitution Rate 0.00036 errors/bp Technique: Reversion
Klenow fragment A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity. Nucleotide Substitution Rate 0.0028 errors/bp Technique: Forward mutational
Klenow fragment A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity. Full length or truncated Full length
Klenow fragment A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity. KM 3.7uM Reaction: Nucleotide incorporation; Substrate: dTTP
Klenow fragment A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity. Kd 42nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Klenow fragment A thumb subdomain mutant of the large fragment of Escherichia coli DNA polymerase I with reduced DNA binding affinity, processivity, and frameshift fidelity. kcat 0.3 /second Reaction: Nucleotide incorporation; Substrate: dTTP

Entry validated by: