Using macromolecular crowding agents to identify weak interactions within DNA replication complexes.

Polymerases:

T4

Topics:

Accessory Proteins/Complexes, Nucleotide Incorporation, Enzyme Substrate Interactions, Methods

One line summary:

Experiments are described in which a macromolecular crowding agent (PEG) is used as a biochemical tool to probe weak specific interactions within the T4 DNA replication system

Status:

new topics/pols set partial results complete validated

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