Using macromolecular crowding agents to identify weak interactions within DNA replication complexes.

Reddy MK, Weitzel SE, Daube SS, Jarvis TC, von Hippel PH
Methods in enzymology (1995), Volume 262, Page 466
PubMed entry

Polymerases:

T4

Topics:

Accessory Proteins/Complexes, Nucleotide Incorporation, Enzyme Substrate Interactions, Methods

One line summary:

Experiments are described in which a macromolecular crowding agent (PEG) is used as a biochemical tool to probe weak specific interactions within the T4 DNA replication system

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