Sensitivity to phosphonoacetic acid: a new phenotype to probe DNA polymerase delta in Saccharomyces cerevisiae.


A mutant allele (pol3-L612M) of the DNA polymerase delta gene in ...
A mutant allele (pol3-L612M) of the DNA polymerase delta gene in Saccharomyces cerevisiae that confers sensitivity to the antiviral drug phosphonoacetic acid (PAA) was constructed. We report that PAA-sensitivity tagging DNA polymerases is a useful method for selectively and reversibly inhibiting one type of DNA polymerase. Our initial studies reveal that replication by the L612M-DNA pol delta requires Rad27 flap endonuclease activity since the pol3-L612M strain is not viable in the absence of RAD27 function. The L612M-DNA pol delta also strongly depends on mismatch repair (MMR). Reduced viability is observed in the absence of any of the core MMR proteins-Msh2, Mlh1, or Pms1-and severe sensitivity to PAA is observed in the absence of the core proteins Msh6 or Exo1, but not Msh3. We propose that pol3-L612M cells need the Rad27 flap endonuclease and MMR complexes composed of Msh2/Msh6, Mlh1/Pms1, and Exo1 for correct processing of Okazaki fragments.



Mutational Analysis, Modulators/Inhibitors, Fidelity, Methods

One line summary:

Construction and characterization of the yeast L612M-DNA polymerase delta that is sensitive to the antiviral drug phosphonoacetic acid.


This is the first example of engineering a mutant DNA polymerase delta in yeast that is sensitive to phosphonoacetic acid. A drug-sensitive DNA polymerase alpha is also presented.


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