High-level overproduction of His-tagged Tth DNA polymerase in Thermus thermophilus.

Moreno R, Haro A, Castellanos A, Berenguer J
Appl Environ Microbiol (2005), Volume 71, Page 591
PubMed entry Full article text Publisher's site

Abstract:

A new plasmid for the overexpression of His-tagged thermozymes in ...
A new plasmid for the overexpression of His-tagged thermozymes in Thermus thermophilus was developed. With this plasmid, soluble and active histidine-tagged DNA polymerase from T. thermophilus was overproduced in larger amounts in the thermophile than in Escherichia coli. The protein purified from the thermophile was active in PCR.

Polymerases:

Tth Pol I

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