The Epstein-Barr virus DNA polymerase transactivates the human immunodeficiency virus type 1 5' long terminal repeat.


We have demonstrated in transient expression assays that the Epstein-Barr virus (EBV) DNA polymerase transactivates expression of the bacterial chloramphenicol acetyltransferase (CAT) gene linked to the human immunodeficiency virus (HIV) type 1 5' long terminal repeat (LTR). The evidence was provided by two sets of experiments. Transfection of Raji cells with HIV LTR-CAT followed by superinfection with EBV resulted in a 150-fold increase in CAT activity. In the presence of viral DNA inhibitor 3'-azido-3'-deoxythymidine (AZT), the CAT activity was inhibited by approximately 70%, suggesting that EBV DNA polymerase was involved in the transactivation of HIV LTR. The direct proof came from the cotransfection of HIV LTR-CAT with expression plasmid containing EBV polymerase gene; depending on the polymerase gene construct cotransfection with both plasmids resulted in a 23- to 38-fold increase of HIV LTR-CAT activity. The interaction between EBV polymerase and HIV may contribute to the role of EBV as a cofactor in the pathogenesis of AIDS.




new topics/pols set partial results complete validated


No results available for this paper.

Entry validated by:

Log in to edit reference All References

Using Polbase tables:


Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).


It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.