Selective inhibition of DNA primase activity associated with DNA polymerase alpha from calf thymus.

Abstract:

The photo-activatable analogs of ATP, 3'-O-(4-benzoyl) benzoic adenosine 5'-triphosphate (BzATP) and 8-azidoadenosine 5'-triphosphate (8-N3-ATP) were used to study the relationship between the polymerase activity and the closely associated primase activity of calf DNA polymerase alpha. A substantial loss of DNA primase activity occurred during pre-incubation and irradiation of DNA polymerase alpha with either BzATP or 8-N3-ATP. In contrast, polymerase activity was only slightly affected. In reactions carried out after pre-incubation with BzATP or 8-N3-ATP in the absence of UV illumination, inhibition was still observed, but it could be reversed by ATP. The specificity of the inhibition for primase activity, plus the ability of ATP to act as a antagonist of BzATP and 8-N3-ATP, suggest that effective interaction of these analogs with the multisubunit polymerase-primase complex is occurring uniquely at the active site of the DNA primase.

Polymerases:

Topics:

Status:

new topics/pols set partial results complete validated

Results:

No results available for this paper.

Entry validated by:

Log in to edit reference All References

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.