Poly(ADP-ribosyl)ation of DNA polymerase beta in vitro.

Ohashi Y, Itaya A, Tanaka Y, Yoshihara K, Kamiya T, Matsukage A
Biochem Biophys Res Commun (1986), Volume 140, Page 666
PubMed entry

Abstract:

DNA polymerase beta purified from bovine thymus is markedly inhibited ...
DNA polymerase beta purified from bovine thymus is markedly inhibited when incubated in a reconstituted poly(ADP-ribosyl)ating reaction system. Analyses of the reaction product synthesized in this system by SDS-polyacrylamide gel electrophoresis and subsequent fluorography of the gel indicated that ADP-ribose is covalently attached to DNA polymerase beta molecule (Mr = 44,000).

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