[DNA-polymerase from sea urchin (Strongylocentrotus intermedius. Embryos].


Using DEAE-cellulose chromatography, three peaks of the DNA-polymerase ...
Using DEAE-cellulose chromatography, three peaks of the DNA-polymerase activity were found in a homogenate of the sea urchin Strongylocentrotus intermedius embryos at stage 32 of the blastomer. The isolation and purification of DNA-polymerase making up the bulk of he DNA-synthesizing activity of the sea urchin embryo cells included fractionation by ammonium sulfate, chromatography on DEAE-cellulose, hydroxyapatite and DNA-cellulose, resulting in a 2750-fold purification of the enzyme. The enzyme activity requires the presence of two-chain DNA activated by pancreatic DNAse, four dNTP and Mg2+. The enzyme is inhibited by a high ionic strength (150 mM KCl or NaCL) and the sulfhydryl reagent--N-ethylmaleimide; the pH optimum is 8.0. The molecular weight of the enzyme as determined by gel-filtration is about 150 000. It is assumed that the enzyme under study can be related to DNA-polymerases of the alpha-type.




new topics/pols set partial results complete validated


No results available for this paper.

Entry validated by:

Using Polbase tables:


Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).


It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.