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Reference: Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I.

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Polymerase	Reference	Property	Result	Context
Klenow fragment	Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I.	Cloned or native	Cloned in E. coli
Klenow fragment	Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I.	Residues Involved in Catalysis of 3-5' Exo	D355, E357, D424, D501
Klenow fragment	Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I.	Tagged	No
Klenow fragment	Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I.	Full length or truncated	Full length
Klenow fragment	Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I.	Other Important Residues	2 Mn ions show metal A and B positions
Klenow fragment	Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I.	Specific Activity	1.07E+04 units/mg	Technique: Polymerase assay (poly[d(AT)]
KF D424A	Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I.	Other Important Residues	Metal B not bound
KF D424A	Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I.	Specific Activity	9300 units/mg	Technique: Polymerase assay (poly[d(AT)]
KF D424A	Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I.	Specific Activity	1.3E-05 units/mg	Technique: Exo activity (arbitrary units) (relative to WT = 1)
KF D355AE357A	Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I.	Other Important Residues	No binding of metals A and B and dNMP
KF D355AE357A	Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I.	Specific Activity	1.11E+04 units/mg	Technique: Polymerase assay (poly[d(AT)]
KF D355AE357A	Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I.	Specific Activity	1.4E-05 units/mg	Technique: Exo activity (arbitrary units) (Relative to WT = 1)