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Reference: Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I.

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Polymerase Reference Property Result Context
Klenow fragment Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I. Cloned or native Cloned in E. coli
Klenow fragment Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I. Residues Involved in Catalysis of 3-5' Exo D355, E357, D424, D501
Klenow fragment Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I. Tagged No
Klenow fragment Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I. Full length or truncated Full length
Klenow fragment Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I. Other Important Residues 2 Mn ions show metal A and B positions
Klenow fragment Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I. Specific Activity 1.07E+04 units/mg Technique: Polymerase assay (poly[d(AT)]
KF D424A Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I. Other Important Residues Metal B not bound
KF D424A Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I. Specific Activity 9300 units/mg Technique: Polymerase assay (poly[d(AT)]
KF D424A Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I. Specific Activity 1.3E-05 units/mg Technique: Exo activity (arbitrary units) (relative to WT = 1)
KF D355AE357A Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I. Other Important Residues No binding of metals A and B and dNMP
KF D355AE357A Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I. Specific Activity 1.11E+04 units/mg Technique: Polymerase assay (poly[d(AT)]
KF D355AE357A Genetic and crystallographic studies of the 3',5'-exonucleolytic site of DNA polymerase I. Specific Activity 1.4E-05 units/mg Technique: Exo activity (arbitrary units) (Relative to WT = 1)

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Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

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It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.