Poly rC-oligo dG specific template activity of mouse viral DNA polymerase detected by isoelectric focusing.


An acid fraction from polyacrylamide gel isoelectric focusing ...
An acid fraction from polyacrylamide gel isoelectric focusing contained only DNA polymerase template activity for (rC)n-(dG)12-18 with little or no activity with (rA)n-(dT)12-18. A factor which stimulated (rC)n-(dG) 12-18 activity was also detected in low molecular weight fractions from G-100 Sephadex chromatography of the purified enzyme. Since (rC)n-(dG)12-18 acitivty is thought to be descriptive of viral DNA polymerase, this stimulation is proposed to be significant for the regulation and specificity of viral DNA synthesis.




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