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Reference: Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity.
Polymerase | Reference | Property | Result | Context |
---|---|---|---|---|
T4 D219A | Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity. | Nucleotide Substitution Rate | 760 Mutation frequency (relative to WT) | Technique: ac forward mutational assay |
T4 D219A | Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity. | Kd | 40nM | Reaction: 3-5' Exonuclease; Substrate: DNA template |
T4 D219A | Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity. | Kd | 10uM | Reaction: Nucleotide incorporation; Substrate: dATP |
T4 D219A | Construction and characterization of a bacteriophage T4 DNA polymerase deficient in 3'-->5' exonuclease activity. | kcat | 3 /second | Reaction: Nucleotide incorporation; Substrate: dATP |
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