Mutator mutations in bacteriophage T4 gene 42 (dHMC hydroxymethylase).

Abstract:

Temperature-sensitive mutations of bacteriophage T4 gene 42 produce diverse effects upon spontaneous mutation rate. G:C vector A:T transition rates are increased, often strongly; frameshift mutation rates are weakly increased; A:T vector G:C transition rates (and perhaps also A:T vector Py:Pu transversion rates) are decreased; and one G:C vector Py:Pu transversion rate is also decreased. These results, together with certain interactions between gene-42 mutator effects and both base analogue mutagenesis and the viral error-prone repair system, suggest that the dHMC hydroxymethylase coded by gene 42 affects mutation rates in a more complex manner than by the simple regulation of the concentration of the DNA precursor dHMCTP.

Polymerases:

T4

Topics:

Mutational Analysis, Fidelity, Accessory Proteins/Complexes

One line summary:

Temperature sensitive mutations of gp42 can results in an increase of mutations, most notably G:C to A:T mutations being significantly increased while A:T to G:C mutations being weakly increased

Status:

new topics/pols set partial results complete validated

Results:

No results available for this paper.

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