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Reference: Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal.

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Polymerase Reference Property Result Context
Bst LF Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Polymerase Catalytic Residue Amino Acids Asp653,Asp830,Glu831
Bst LF Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. 3-5' Exonuclease (proofreading) No
Bst LF Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Cloned or native Cloned in E. coli
Bst LF Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. 5-3' Exonuclease Yes
Bst LF Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Full length or truncated Truncated
Bst LF Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Amino Acids Contacting Template Tyr714
Bst LF Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Processivity 111bp
Bst LF Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Specific Activity 1.5E+05 units/mg Technique: Polymerase Assay (calf thymus DNA)
Bst LF Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Specific Activity 4.9E+05 units/mg Technique: Polymerase Assay (M13 DNA)
Bst LF Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. KM 13uM Reaction: Nucleotide incorporation; Substrate: dNTPs
Bst LF Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. KM 3.4nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Bst LF Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. kcat 191.2 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Bst LF Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Describe truncation contains aa 297-876
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. 3-5' Exonuclease (proofreading) No
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Full length or truncated Full length
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Processivity 10bp
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Specific Activity 2.2E+04 units/mg Technique: Polymerase Assay (calf thymus DNA)
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Specific Activity 4.9E+04 units/mg Technique: Polymerase Assay (M13 DNA)
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. KM 3.5nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. KM 24uM Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. kcat 46.6 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Klenow fragment Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Polymerase Catalytic Residue Amino Acids Asp705, Asp882, and Glu883
Klenow fragment Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. 3-5' Exonuclease (proofreading) Yes
Klenow fragment Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Residues Involved in Catalysis of 3-5' Exo Asp355, Glu357, Asp424, and Asp501
Klenow fragment Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Processivity 70bp
Klenow fragment Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Specific Activity 5500 units/mg Technique: Polymerase Assay (M13 DNA)
Klenow fragment Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Specific Activity 9700 units/mg Technique: Polymerase Assay (calf thymus DNA)
Klenow fragment Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. KM 2.3uM Reaction: Nucleotide incorporation; Substrate: dNTPs
Klenow fragment Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. KM 1.8nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Klenow fragment Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. kcat 5.2 /second Reaction: Nucleotide incorporation; Substrate: dNTPs

Version:20241007

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.