Bst LF

From Bacillus stearothermophilus (aliases:Bst (large fragment), bstlf)
show help

Welcome to the Polymerase Page

This page presents all the information in Polbase for Bst LF.


At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.


If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.

External links:

Polbase polymerases are linked to UniProt and Genbank where possible. The UniProt search link directs you to a pre-constructed UniProt search containing relevant terms.


All references that refer to this Polymerase are listed with their topics and paper's title in the References table.


All the results for this polymerase can be examined by following the "Results" link at the bottom of the page.

Help icons:

The show help symbol in the upper-right corner of the page links to this help text. The question mark icon is used everywhere to indicate that help is available.

Mutant of: Bst ()

Bst LF is a family A enzyme from Bacillus stearothermophilus.

N-terminal 5'-3' exo- domain removed

Selected Properties for Bst LF:

3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
No (3) no consensus no data no data no data
Year Authors Title Results by property
2012 Sarah E Graham, FatimaSultana Syeda, G Andrés Cisneros Computational prediction of residues ...
2011 Eugene Y Wu, Lorena S Beese The structure of a high fidelity DNA ...
2008 Suse Broyde, Liming Wang, Olga Rechkoblit, Nicholas E Geacintov, Dinshaw J Patel Lesion processing: high-fidelity vers...
2004 Gerald W Hsu, Matthias Ober, Thomas Carell, Lorena S Beese Error-prone replication of oxidativel...
2004 Daisuke Shirane, Kohtaroh Sugao, Shigeyuki Namiki, Mao Tanabe, Masamitsu Iino, Kenzo Hirose Enzymatic production of RNAi librarie...
2004 Sean J Johnson, Lorena S Beese Structures of mismatch replication er...
2003 Sean J Johnson, Jeffrey S Taylor, Lorena S Beese Processive DNA synthesis observed in ... Cloned or native,Tagged,Full length or truncated,Describe truncation,All properties
2001 Holly H Hogrefe, J Cline, A E Lovejoy, K B Nielson DNA polymerases from hyperthermophiles. RT,Specific Activity,Processivity,Strand Displacement,3-5' exo,5-3' exo,KM,kcat,All properties
1998 M A Milla, P A Spears, R E Pearson, G T Walker Use of the restriction enzyme AvaI an... Application name,All properties
1998 James R Kiefer, C Mao, J C Braman, Lorena S Beese Visualizing DNA replication in a cata... Cloned or native,Full length or truncated,Specific Activity,Processivity,3-5' exo,5-3' exo,KM,kcat,Describe truncation,Amino Acids Contacting Template,Polymerase Catalytic Residue Amino Acids,All properties
1997 James R Kiefer, C Mao, Connie J Hansen, S L Basehore, Holly H Hogrefe, J C Braman, Lorena S Beese Crystal structure of a thermostable B... KM,kcat,Describe truncation,All properties
1996 J M Aliotta, J J Pelletier, J L Ware, L S Moran, J S Benner, H Kong Thermostable Bst DNA polymerase I lac... Cloned or native,Tagged,Tag Name,Full length or truncated,Specific Activity,3-5' exo,5-3' exo,Describe truncation,Molecular Weight,All properties
Name 3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
Bst LF (D329A)
Bst LF (D329AD598A)
Bst LF D329AY714S
Bst LF (D598A)
Bst LF D598AF710Y
Bst LF V713P

Using Polbase tables:


Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).


It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.