Preparation and enzymatic recognition of guanine lesions induced by nitrogen oxides.

Masaoka A, Terato H, Honsho A, Ohyama Y, Suzuki T, Yamada M, Makino K, Ide H
Nucleic acids symposium series (2000), Page 87
PubMed entry

Abstract:

Xanthine (Xan) and oxanine (Oxa) are the major deamination products of ...
Xanthine (Xan) and oxanine (Oxa) are the major deamination products of guanine formed by the treatment with nitrogen oxides (e.g., NO and HNO2). In this study, 2'-deoxyribonucleoside 5'-triphosphates of Xan and Oxa were prepared by the NaNO2 treatment of dGTP. These modified nucleotides were incorporated into oligonucleotides by DNA polymerase reactions. The repair activities of various DNA N-glycosylases for Xan and Oxa were examined using these substrates.

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