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Reference: The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.

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Polymerase	Reference	Property	Result	Context
Klenow fragment	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	k_cat	0.12 /second	Reaction: 3-5' Exonuclease; Technique: Steady State (ssDNA: poly(dA)); Experimental conditions: Temp (37°C), Mg++ (8mM), pH (pH 7.5)
Klenow fragment	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	k_cat	0.09 /second	Reaction: 3-5' Exonuclease; Technique: Steady State (ssDNA: poly(dT)); Experimental conditions: Temp (37°C), Mg++ (6mM), pH (pH 7.5)
Klenow fragment	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	K_M	0.56uM	Reaction: 3-5' Exonuclease; Technique: Steady State (ssDNA: poly(dT)); Experimental conditions: Temp (37°C), Mg++ (6mM), pH (pH 7.5)
Klenow fragment	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	Specific Activity	9000 units/mg	Technique: Polymerase assay (poly[d(AT)]
Klenow fragment	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	Full length or truncated	Full length
Klenow fragment	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	Tagged	No
Klenow fragment	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	Cloned or native	Cloned in E. coli
Klenow fragment	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	3-5' Exonuclease (proofreading)	Yes
KF D355A	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	k_cat	8.3E-08 /second	Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA substrate, assuming WT has rate 0.001/s); Experimental conditions: Temp (37°C), Mg++ (6mM), pH (pH 7.5)
KF D355A	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	Specific Activity	1.3E+04 units/mg	Technique: Polymerase assay (poly[d(AT)]
KF E357A	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	k_cat	1.8E-06 /second	Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: Temp (37°C), Mg++ (6mM), pH (pH 7.5)
KF E357A	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	Specific Activity	1.2E+04 units/mg	Technique: Polymerase assay (poly[d(AT)]
KF L361A	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	k_cat	4E-05 /second	Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: Temp (37°C), Mg++ (6mM), pH (pH 7.5)
KF L361A	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	k_cat	0.044 /second	Reaction: 3-5' Exonuclease; Technique: Steady State (ssDNA, poly(dA)); Experimental conditions: Temp (37°C), Mg++ (8mM), pH (pH 7.5)
KF L361A	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	Specific Activity	9000 units/mg	Technique: Polymerase assay (poly[d(AT)]
KF L361M	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	k_cat	8.3E-05 /second	Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: Temp (37°C), Mg++ (6mM), pH (pH 7.5)
KF L361M	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	Specific Activity	1.1E+04 units/mg	Technique: Polymerase assay (poly[d(AT)]
KF D424A	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	k_cat	1.3E-08 /second	Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: Temp (37°C), pH (pH 7.5)
KF D424A	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	Specific Activity	9000 units/mg	Technique: Polymerase assay (poly[d(AT)]
KF D424E	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	k_cat	4E-05 /second	Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: Temp (37°C), Mg++ (6mM), pH (pH 7.5)
KF D424E	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	k_cat	0.01 /second	Reaction: 3-5' Exonuclease; Technique: Steady State (ssDNA, poly(dA)); Experimental conditions: Temp (37°C), Mg++ (8mM), pH (pH 7.5)
KF D424E	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	Specific Activity	1.1E+04 units/mg	Technique: Polymerase assay (poly[d(AT)]
KF D424N	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	k_cat	2.5E-08 /second	Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: Temp (37°C), Mg++ (6mM), pH (pH 7.5)
KF D424N	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	Specific Activity	1.2E+04 units/mg	Technique: Polymerase assay (poly[d(AT)]
KF F473A	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	k_cat	2.9E-07 /second	Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: Temp (37°C), pH (pH 7.5)
KF F473A	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	Specific Activity	1E+04 units/mg	Technique: Polymerase assay (poly[d(AT)]
KF Y497F	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	k_cat	4.3E-05 /second	Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: Temp (37°C), Mg++ (6mM), pH (pH 7.5)
KF Y497F	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	k_cat	0.0019 /second	Reaction: 3-5' Exonuclease; Technique: Steady State (ssDNA, poly(dA)); Experimental conditions: Temp (37°C), Mg++ (8mM), pH (pH 7.5)
KF Y497F	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	Specific Activity	1.1E+04 units/mg	Technique: Polymerase assay (poly[d(AT)]
KF D501A	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	k_cat	7.5E-08 /second	Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: Temp (37°C), Mg++ (6mM), pH (pH 7.5)
KF D501A	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	Specific Activity	1.2E+04 units/mg	Technique: Polymerase assay (poly[d(AT)]
KF D510E	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	k_cat	5.6E-06 /second	Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: Temp (37°C), Mg++ (6mM), pH (pH 7.5)
KF D510E	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	Specific Activity	1E+04 units/mg	Technique: Polymerase assay (poly[d(AT)]
KF D510N	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	k_cat	0.0005 /second	Reaction: 3-5' Exonuclease; Technique: Steady State (dsDNA, assuming WT has rate 0.001/s); Experimental conditions: Temp (37°C), pH (pH 7.5)
KF D510N	The 3'-5' exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.	Specific Activity	1E+04 units/mg	Technique: Polymerase assay (poly[d(AT)]