show help

Results for:

Polymerase: Taq pol I

Welcome to the results summary page

This page presents all the results for a polymerase, property or reference. To find more information about a polymerase or reference, follow the relevant link.

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.

Help icons:

The show help symbol in the upper-right corner of the page links to this help text. The question mark icon is used everywhere to indicate that help is available.

Polymerase Reference Property Result Context
Taq pol I Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Nucleotide Substitution Rate 0.0002 errors/bp Technique: Sequencing
Taq pol I Fidelity of DNA polymerases in DNA amplification. Nucleotide Substitution Rate 0.00021 errors/bp Technique: DGGE
Taq pol I Fidelity of Thermococcus litoralis DNA polymerase (Vent) in PCR determined by denaturing gradient gel electrophoresis. 3-5' Exonuclease (proofreading) No
Taq pol I Fidelity of Thermococcus litoralis DNA polymerase (Vent) in PCR determined by denaturing gradient gel electrophoresis. Nucleotide Substitution Rate 8.9E-05 errors/bp
Taq pol I Fidelity of Thermococcus litoralis DNA polymerase (Vent) in PCR determined by denaturing gradient gel electrophoresis. Application name PCR
Taq pol I Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR. Nucleotide Substitution Rate 0.00018 errors/bp Technique: Blue/white screening (0M Betaine, pWB407 template)
Taq pol I Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR. Nucleotide Substitution Rate 0.00021 errors/bp Technique: Blue/white screening (1.3M Betaine, pWB407 template)
Taq pol I Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. 3-5' Exonuclease (proofreading) No
Taq pol I Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. Frameshift Error Rate 2.44E-05 errors/bp
Taq pol I Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. Nucleotide Substitution Rate 0.000111 errors/bp
Taq pol I Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. Full length or truncated Full length
Taq pol I Crystal structure of a thermostable Bacillus DNA polymerase I large fragment at 2.1 A resolution. Processivity 10bp
Taq pol I Crystal structure of a thermostable Bacillus DNA polymerase I large fragment at 2.1 A resolution. Specific Activity 2.2E+04 units/mg
Taq pol I Crystal structure of a thermostable Bacillus DNA polymerase I large fragment at 2.1 A resolution. KM 3.5nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Technique: Filter binding ; Experimental conditions: Temp (72°C)
Taq pol I Crystal structure of a thermostable Bacillus DNA polymerase I large fragment at 2.1 A resolution. KM 24uM Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Filter binding ; Experimental conditions: Temp (72°C)
Taq pol I Crystal structure of a thermostable Bacillus DNA polymerase I large fragment at 2.1 A resolution. kcat 46.6 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Filter binding (16.5nM primed M13); Experimental conditions: Temp (72°C)
Taq pol I Incorporation of reporter-labeled nucleotides by DNA polymerases. 3-5' Exonuclease (proofreading) No
Taq pol I Incorporation of reporter-labeled nucleotides by DNA polymerases. Incorporation of non-standard nucleotides Unspecified Nucleotide analog: Fluorescent dNTPs
Taq pol I Incorporation of reporter-labeled nucleotides by DNA polymerases. Incorporation of non-standard nucleotides Unspecified Nucleotide analog: Biotin-dNTPs
Taq pol I Incorporation of reporter-labeled nucleotides by DNA polymerases. Incorporation of non-standard nucleotides Unspecified
Taq pol I Incorporation of reporter-labeled nucleotides by DNA polymerases. Cloned or native Unspecified
Taq pol I Incorporation of reporter-labeled nucleotides by DNA polymerases. 5-3' Exonuclease Unspecified
Taq pol I Incorporation of reporter-labeled nucleotides by DNA polymerases. Tagged Unspecified
Taq pol I Incorporation of reporter-labeled nucleotides by DNA polymerases. Full length or truncated Unspecified
Taq pol I Incorporation of reporter-labeled nucleotides by DNA polymerases. Application name PCR
Taq pol I Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis Overall Error Rate 1.213E-05 errors/bp
Taq pol I Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis Vmax 80 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase. 5-3' Exonuclease Yes
Taq pol I Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase. Nucleotide Substitution Rate 0.00192 errors/bp
Taq pol I Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase. Processivity 80kb
Taq pol I Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase. Specific Activity 2224 units/mg Technique: Polymerase Assay (M13 DNA) (With thioredoxin)
Taq pol I Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase. Specific Activity 2218 units/mg Technique: Polymerase Assay (M13 DNA) (Without thioredoxin)
Taq pol I Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis. Vent DNA polymerase, steady state kinetics, thermal stability, processivity, strand displacement, and exonuclease activities. Full length or truncated Full length
Taq pol I Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis. Vent DNA polymerase, steady state kinetics, thermal stability, processivity, strand displacement, and exonuclease activities. Processivity 42bp
Taq pol I Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis. Vent DNA polymerase, steady state kinetics, thermal stability, processivity, strand displacement, and exonuclease activities. KM 16uM Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Filter binding ; Experimental conditions: Temp (72°C)
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. 3-5' Exonuclease (proofreading) No
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Full length or truncated Full length
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Processivity 10bp
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Specific Activity 2.2E+04 units/mg Technique: Polymerase Assay (calf thymus DNA)
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Specific Activity 4.9E+04 units/mg Technique: Polymerase Assay (M13 DNA)
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. KM 3.5nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. KM 24uM Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. kcat 46.6 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. Molecular Weight 9.4E+04 Dalton
Taq pol I High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. Cloned or native Cloned in E. coli
Taq pol I High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. 5-3' Exonuclease Yes
Taq pol I High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. Full length or truncated Full length
Taq pol I High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. Specific Activity 2.92E+05 Other
Taq pol I High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. Vmax 150 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Temp (75°C)
Taq pol I Thermostable DNA polymerases. Molecular Weight 9.39E+04 Dalton Technique: Sequence
Taq pol I Thermostable DNA polymerases. 3-5' Exonuclease (proofreading) No
Taq pol I Thermostable DNA polymerases. 5-3' Exonuclease Yes
Taq pol I PCR fidelity of pfu DNA polymerase and other thermostable DNA polymerases. Overall Error Rate 8E-06 errors/bp
Taq pol I Optimization of the polymerase chain reaction with regard to fidelity: modified T7, Taq, and vent DNA polymerases. Overall Error Rate 7.2E-05 errors/bp
Taq pol I Optimization of the polymerase chain reaction with regard to fidelity: modified T7, Taq, and vent DNA polymerases. Overall Error Rate 0.0002 errors/bp
Taq pol I DNA polymerases from hyperthermophiles. Reverse Transcriptase Activity Yes
Taq pol I DNA polymerases from hyperthermophiles. 3-5' Exonuclease (proofreading) No
Taq pol I DNA polymerases from hyperthermophiles. 5-3' Exonuclease Yes
Taq pol I DNA polymerases from hyperthermophiles. Overall Error Rate 8E-06 errors/bp
Taq pol I DNA polymerases from hyperthermophiles. Processivity 10bp
Taq pol I DNA polymerases from hyperthermophiles. Specific Activity 2.2 units/mg Technique: Polymerase Assay (calf thymus DNA)
Taq pol I DNA polymerases from hyperthermophiles. Specific Activity 4.9 units/mg Technique: Polymerase Assay (M13 DNA)
Taq pol I DNA polymerases from hyperthermophiles. KM 24uM Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I DNA polymerases from hyperthermophiles. KM 4nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Taq pol I DNA polymerases from hyperthermophiles. Strand Displacement Yes
Taq pol I DNA polymerases from hyperthermophiles. kcat 2800 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. 3-5' Exonuclease (proofreading) No
Taq pol I High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. Cloned or native Cloned in E. coli
Taq pol I High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. Frameshift Error Rate 5.26E-05 errors/bp Technique: Reversion (Low fidelity conditions)
Taq pol I High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. Frameshift Error Rate 4.76E-06 errors/bp Technique: Reversion (High fidelity conditions)
Taq pol I High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. Nucleotide Substitution Rate 0.0003125 errors/bp Technique: M13mp2 opal codon reversion assay (Low fidelity conditions)
Taq pol I High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. Nucleotide Substitution Rate 3.33E-06 errors/bp Technique: M13mp2 opal codon reversion assay (High fidelity conditions)
Taq pol I High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. Full length or truncated Full length
Taq pol I Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I. Cloned or native Cloned in yeast
Taq pol I Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I. Tagged Yes
Taq pol I Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I. Tag Name intein
Taq pol I Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I. Full length or truncated Full length
Taq pol I Characterization of the 5' to 3' nuclease activity of Thermus aquaticus DNA polymerase on fluorogenic double-stranded probes. 3-5' Exonuclease (proofreading) No
Taq pol I Characterization of the 5' to 3' nuclease activity of Thermus aquaticus DNA polymerase on fluorogenic double-stranded probes. 5-3' Exonuclease Yes
Taq pol I A plasmid-based lacZα gene assay for DNA polymerase fidelity measurement. Overall Error Rate 1E-05 errors/bp Technique: Blue/white screening (pSJ2)
Taq pol I A plasmid-based lacZα gene assay for DNA polymerase fidelity measurement. Overall Error Rate 1.1E-05 errors/bp

Version:20241218

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.