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Polymerase: Taq pol I

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Polymerase Reference Property Result Context
Taq pol I Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Nucleotide Substitution Rate 0.0002 errors/bp Technique: Sequencing
Taq pol I Fidelity of DNA polymerases in DNA amplification. Nucleotide Substitution Rate 0.00021 errors/bp Technique: DGGE
Taq pol I Fidelity of Thermococcus litoralis DNA polymerase (Vent) in PCR determined by denaturing gradient gel electrophoresis. 3-5' Exonuclease (proofreading) No
Taq pol I Fidelity of Thermococcus litoralis DNA polymerase (Vent) in PCR determined by denaturing gradient gel electrophoresis. Nucleotide Substitution Rate 8.9E-05 errors/bp
Taq pol I Fidelity of Thermococcus litoralis DNA polymerase (Vent) in PCR determined by denaturing gradient gel electrophoresis. Application name PCR
Taq pol I Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR. Nucleotide Substitution Rate 0.00018 errors/bp Technique: Blue/white screening (0M Betaine, pWB407 template)
Taq pol I Cold-sensitive mutants of Taq DNA polymerase provide a hot start for PCR. Nucleotide Substitution Rate 0.00021 errors/bp Technique: Blue/white screening (1.3M Betaine, pWB407 template)
Taq pol I Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. 3-5' Exonuclease (proofreading) No
Taq pol I Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. Frameshift Error Rate 2.44E-05 errors/bp
Taq pol I Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. Nucleotide Substitution Rate 0.000111 errors/bp
Taq pol I Fidelity of DNA synthesis by the Thermus aquaticus DNA polymerase. Full length or truncated Full length
Taq pol I Crystal structure of a thermostable Bacillus DNA polymerase I large fragment at 2.1 A resolution. Processivity 10bp
Taq pol I Crystal structure of a thermostable Bacillus DNA polymerase I large fragment at 2.1 A resolution. Specific Activity 2.2E+04 units/mg
Taq pol I Crystal structure of a thermostable Bacillus DNA polymerase I large fragment at 2.1 A resolution. KM 3.5nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Technique: Filter binding ; Experimental conditions: Temp (72°C)
Taq pol I Crystal structure of a thermostable Bacillus DNA polymerase I large fragment at 2.1 A resolution. KM 24uM Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Filter binding ; Experimental conditions: Temp (72°C)
Taq pol I Crystal structure of a thermostable Bacillus DNA polymerase I large fragment at 2.1 A resolution. kcat 46.6 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Filter binding (16.5nM primed M13); Experimental conditions: Temp (72°C)
Taq pol I Incorporation of reporter-labeled nucleotides by DNA polymerases. 3-5' Exonuclease (proofreading) No
Taq pol I Incorporation of reporter-labeled nucleotides by DNA polymerases. Incorporation of non-standard nucleotides Unspecified Nucleotide analog: Fluorescent dNTPs
Taq pol I Incorporation of reporter-labeled nucleotides by DNA polymerases. Incorporation of non-standard nucleotides Unspecified Nucleotide analog: Biotin-dNTPs
Taq pol I Incorporation of reporter-labeled nucleotides by DNA polymerases. Incorporation of non-standard nucleotides Unspecified
Taq pol I Incorporation of reporter-labeled nucleotides by DNA polymerases. Cloned or native Unspecified
Taq pol I Incorporation of reporter-labeled nucleotides by DNA polymerases. 5-3' Exonuclease Unspecified
Taq pol I Incorporation of reporter-labeled nucleotides by DNA polymerases. Tagged Unspecified
Taq pol I Incorporation of reporter-labeled nucleotides by DNA polymerases. Full length or truncated Unspecified
Taq pol I Incorporation of reporter-labeled nucleotides by DNA polymerases. Application name PCR
Taq pol I Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis Overall Error Rate 1.213E-05 errors/bp
Taq pol I Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis Vmax 80 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase. 5-3' Exonuclease Yes
Taq pol I Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase. Nucleotide Substitution Rate 0.00192 errors/bp
Taq pol I Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase. Processivity 80kb
Taq pol I Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase. Specific Activity 2224 units/mg Technique: Polymerase Assay (M13 DNA) (With thioredoxin)
Taq pol I Insertion of the T3 DNA polymerase thioredoxin binding domain enhances the processivity and fidelity of Taq DNA polymerase. Specific Activity 2218 units/mg Technique: Polymerase Assay (M13 DNA) (Without thioredoxin)
Taq pol I Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis. Vent DNA polymerase, steady state kinetics, thermal stability, processivity, strand displacement, and exonuclease activities. Full length or truncated Full length
Taq pol I Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis. Vent DNA polymerase, steady state kinetics, thermal stability, processivity, strand displacement, and exonuclease activities. Processivity 42bp
Taq pol I Characterization of a DNA polymerase from the hyperthermophile archaea Thermococcus litoralis. Vent DNA polymerase, steady state kinetics, thermal stability, processivity, strand displacement, and exonuclease activities. KM 16uM Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Filter binding ; Experimental conditions: Temp (72°C)
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. 3-5' Exonuclease (proofreading) No
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Full length or truncated Full length
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Processivity 10bp
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Specific Activity 2.2E+04 units/mg Technique: Polymerase Assay (calf thymus DNA)
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. Specific Activity 4.9E+04 units/mg Technique: Polymerase Assay (M13 DNA)
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. KM 3.5nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. KM 24uM Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I Visualizing DNA replication in a catalytically active Bacillus DNA polymerase crystal. kcat 46.6 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. Molecular Weight 9.4E+04 Dalton
Taq pol I High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. Cloned or native Cloned in E. coli
Taq pol I High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. 5-3' Exonuclease Yes
Taq pol I High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. Full length or truncated Full length
Taq pol I High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. Specific Activity 2.92E+05 Other
Taq pol I High-level expression, purification, and enzymatic characterization of full-length Thermus aquaticus DNA polymerase and a truncated form deficient in 5' to 3' exonuclease activity. Vmax 150 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Temp (75°C)
Taq pol I Thermostable DNA polymerases. Molecular Weight 9.39E+04 Dalton Technique: Sequence
Taq pol I Thermostable DNA polymerases. 3-5' Exonuclease (proofreading) No
Taq pol I Thermostable DNA polymerases. 5-3' Exonuclease Yes
Taq pol I PCR fidelity of pfu DNA polymerase and other thermostable DNA polymerases. Overall Error Rate 8E-06 errors/bp
Taq pol I Optimization of the polymerase chain reaction with regard to fidelity: modified T7, Taq, and vent DNA polymerases. Overall Error Rate 7.2E-05 errors/bp
Taq pol I Optimization of the polymerase chain reaction with regard to fidelity: modified T7, Taq, and vent DNA polymerases. Overall Error Rate 0.0002 errors/bp
Taq pol I DNA polymerases from hyperthermophiles. Reverse Transcriptase Activity Yes
Taq pol I DNA polymerases from hyperthermophiles. 3-5' Exonuclease (proofreading) No
Taq pol I DNA polymerases from hyperthermophiles. 5-3' Exonuclease Yes
Taq pol I DNA polymerases from hyperthermophiles. Overall Error Rate 8E-06 errors/bp
Taq pol I DNA polymerases from hyperthermophiles. Processivity 10bp
Taq pol I DNA polymerases from hyperthermophiles. Specific Activity 2.2 units/mg Technique: Polymerase Assay (calf thymus DNA)
Taq pol I DNA polymerases from hyperthermophiles. Specific Activity 4.9 units/mg Technique: Polymerase Assay (M13 DNA)
Taq pol I DNA polymerases from hyperthermophiles. KM 24uM Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I DNA polymerases from hyperthermophiles. KM 4nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Taq pol I DNA polymerases from hyperthermophiles. Strand Displacement Yes
Taq pol I DNA polymerases from hyperthermophiles. kcat 2800 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. 3-5' Exonuclease (proofreading) No
Taq pol I High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. Cloned or native Cloned in E. coli
Taq pol I High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. Frameshift Error Rate 5.26E-05 errors/bp Technique: Reversion (Low fidelity conditions)
Taq pol I High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. Frameshift Error Rate 4.76E-06 errors/bp Technique: Reversion (High fidelity conditions)
Taq pol I High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. Nucleotide Substitution Rate 0.0003125 errors/bp Technique: M13mp2 opal codon reversion assay (Low fidelity conditions)
Taq pol I High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. Nucleotide Substitution Rate 3.33E-06 errors/bp Technique: M13mp2 opal codon reversion assay (High fidelity conditions)
Taq pol I High fidelity DNA synthesis by the Thermus aquaticus DNA polymerase. Full length or truncated Full length
Taq pol I Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I. Cloned or native Cloned in yeast
Taq pol I Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I. Tagged Yes
Taq pol I Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I. Tag Name intein
Taq pol I Nucleotide sequence of the Escherichia coli polA gene and primary structure of DNA polymerase I. Full length or truncated Full length
Taq pol I Characterization of the 5' to 3' nuclease activity of Thermus aquaticus DNA polymerase on fluorogenic double-stranded probes. 3-5' Exonuclease (proofreading) No
Taq pol I Characterization of the 5' to 3' nuclease activity of Thermus aquaticus DNA polymerase on fluorogenic double-stranded probes. 5-3' Exonuclease Yes
Taq pol I A plasmid-based lacZα gene assay for DNA polymerase fidelity measurement. Overall Error Rate 1E-05 errors/bp Technique: Blue/white screening (pSJ2)
Taq pol I A plasmid-based lacZα gene assay for DNA polymerase fidelity measurement. Overall Error Rate 1.1E-05 errors/bp

Version:20241007

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Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

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