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Polymerase: Taq pol I

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Polymerase Reference Property Result Context
Taq pol I Saiki RK1988 Nucleotide Substitution Rate 0.0002 errors/bp Technique: Sequencing
Taq pol I Keohavong P1989 Nucleotide Substitution Rate 0.00021 errors/bp Technique: DGGE
Taq pol I Cariello NF1991 3-5' Exonuclease (proofreading) No
Taq pol I Cariello NF1991 Nucleotide Substitution Rate 8.9E-05 errors/bp
Taq pol I Cariello NF1991 Application name PCR
Taq pol I Kermekchiev MB2003 Nucleotide Substitution Rate 0.00018 errors/bp Technique: Blue/white screening (0M Betaine, pWB407 template)
Taq pol I Kermekchiev MB2003 Nucleotide Substitution Rate 0.00021 errors/bp Technique: Blue/white screening (1.3M Betaine, pWB407 template)
Taq pol I Tindall KR1988 3-5' Exonuclease (proofreading) No
Taq pol I Tindall KR1988 Frameshift Error Rate 2.44E-05 errors/bp
Taq pol I Tindall KR1988 Nucleotide Substitution Rate 0.000111 errors/bp
Taq pol I Tindall KR1988 Full length or truncated Full length
Taq pol I Kiefer JR1997 Processivity 10bp
Taq pol I Kiefer JR1997 Specific Activity 2.2E+04 units/mg
Taq pol I Kiefer JR1997 KM 3.5nM Reaction: Polymerase-DNA interaction; Substrate: DNA template; Technique: Filter binding ; Experimental conditions: Temp (72°C)
Taq pol I Kiefer JR1997 KM 24uM Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Filter binding ; Experimental conditions: Temp (72°C)
Taq pol I Kiefer JR1997 kcat 46.6 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Filter binding (16.5nM primed M13); Experimental conditions: Temp (72°C)
Taq pol I Anderson JP2005 3-5' Exonuclease (proofreading) No
Taq pol I Anderson JP2005 Incorporation of non-standard nucleotides Unspecified Nucleotide analog: Fluorescent dNTPs
Taq pol I Anderson JP2005 Incorporation of non-standard nucleotides Unspecified Nucleotide analog: Biotin-dNTPs
Taq pol I Anderson JP2005 Incorporation of non-standard nucleotides Unspecified
Taq pol I Anderson JP2005 Cloned or native Unspecified
Taq pol I Anderson JP2005 5-3' Exonuclease Unspecified
Taq pol I Anderson JP2005 Tagged Unspecified
Taq pol I Anderson JP2005 Full length or truncated Unspecified
Taq pol I Anderson JP2005 Application name PCR
Taq pol I Kwon Suk-Tae2009 Overall Error Rate 1.213E-05 errors/bp
Taq pol I Kwon Suk-Tae2009 Vmax 80 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I Davidson JF2003 5-3' Exonuclease Yes
Taq pol I Davidson JF2003 Nucleotide Substitution Rate 0.00192 errors/bp
Taq pol I Davidson JF2003 Processivity 80kb
Taq pol I Davidson JF2003 Specific Activity 2224 units/mg Technique: Polymerase Assay (M13 DNA) (With thioredoxin)
Taq pol I Davidson JF2003 Specific Activity 2218 units/mg Technique: Polymerase Assay (M13 DNA) (Without thioredoxin)
Taq pol I Kong H1993 Full length or truncated Full length
Taq pol I Kong H1993 Processivity 42bp
Taq pol I Kong H1993 KM 16uM Reaction: Nucleotide incorporation; Substrate: dNTPs; Technique: Filter binding ; Experimental conditions: Temp (72°C)
Taq pol I Kiefer JR1998 3-5' Exonuclease (proofreading) No
Taq pol I Kiefer JR1998 Full length or truncated Full length
Taq pol I Kiefer JR1998 Processivity 10bp
Taq pol I Kiefer JR1998 Specific Activity 2.2E+04 units/mg Technique: Polymerase Assay (calf thymus DNA)
Taq pol I Kiefer JR1998 Specific Activity 4.9E+04 units/mg Technique: Polymerase Assay (M13 DNA)
Taq pol I Kiefer JR1998 KM 3.5nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Taq pol I Kiefer JR1998 KM 24uM Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I Kiefer JR1998 kcat 46.6 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I Lawyer FC1993 Molecular Weight 9.4E+04 Dalton
Taq pol I Lawyer FC1993 Cloned or native Cloned in E. coli
Taq pol I Lawyer FC1993 5-3' Exonuclease Yes
Taq pol I Lawyer FC1993 Full length or truncated Full length
Taq pol I Lawyer FC1993 Specific Activity 2.92E+05 Other
Taq pol I Lawyer FC1993 Vmax 150 /second Reaction: Nucleotide incorporation; Substrate: dNTPs; Experimental conditions: Temp (75°C)
Taq pol I Perler FB1996 Molecular Weight 9.39E+04 Dalton Technique: Sequence
Taq pol I Perler FB1996 3-5' Exonuclease (proofreading) No
Taq pol I Perler FB1996 5-3' Exonuclease Yes
Taq pol I Cline J1996 Overall Error Rate 8E-06 errors/bp
Taq pol I Ling LL1991 Overall Error Rate 7.2E-05 errors/bp
Taq pol I Ling LL1991 Overall Error Rate 0.0002 errors/bp
Taq pol I Hogrefe HH2001 Reverse Transcriptase Activity Yes
Taq pol I Hogrefe HH2001 3-5' Exonuclease (proofreading) No
Taq pol I Hogrefe HH2001 5-3' Exonuclease Yes
Taq pol I Hogrefe HH2001 Overall Error Rate 8E-06 errors/bp
Taq pol I Hogrefe HH2001 Processivity 10bp
Taq pol I Hogrefe HH2001 Specific Activity 2.2 units/mg Technique: Polymerase Assay (calf thymus DNA)
Taq pol I Hogrefe HH2001 Specific Activity 4.9 units/mg Technique: Polymerase Assay (M13 DNA)
Taq pol I Hogrefe HH2001 KM 24uM Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I Hogrefe HH2001 KM 4nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Taq pol I Hogrefe HH2001 Strand Displacement Yes
Taq pol I Hogrefe HH2001 kcat 2800 /minute Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I Eckert KA1990 3-5' Exonuclease (proofreading) No
Taq pol I Eckert KA1990 Cloned or native Cloned in E. coli
Taq pol I Eckert KA1990 Frameshift Error Rate 5.26E-05 errors/bp Technique: Reversion (Low fidelity conditions)
Taq pol I Eckert KA1990 Frameshift Error Rate 4.76E-06 errors/bp Technique: Reversion (High fidelity conditions)
Taq pol I Eckert KA1990 Nucleotide Substitution Rate 0.0003125 errors/bp Technique: M13mp2 opal codon reversion assay (Low fidelity conditions)
Taq pol I Eckert KA1990 Nucleotide Substitution Rate 3.33E-06 errors/bp Technique: M13mp2 opal codon reversion assay (High fidelity conditions)
Taq pol I Eckert KA1990 Full length or truncated Full length
Taq pol I Joyce CM1982 Cloned or native Cloned in yeast
Taq pol I Joyce CM1982 Tagged Yes
Taq pol I Joyce CM1982 Tag Name intein
Taq pol I Joyce CM1982 Full length or truncated Full length
Taq pol I Huang Q2009 3-5' Exonuclease (proofreading) No
Taq pol I Huang Q2009 5-3' Exonuclease Yes
Taq pol I Keith BJ2013 Overall Error Rate 1E-05 errors/bp Technique: Blue/white screening (pSJ2)
Taq pol I Keith BJ2013 Overall Error Rate 1.1E-05 errors/bp

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.