Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis

Polymerases:

Topics:

Historical Protein Properties (MW, pI, ...), Kinetic Parameters, Fidelity, Nucleotide Incorporation, Exonuclease Activity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Pfu Kwon Suk-Tae2009 Overall Error Rate 2.18E-06 errors/bp
Pfu Kwon Suk-Tae2009 Vmax 21 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I Kwon Suk-Tae2009 Overall Error Rate 1.213E-05 errors/bp
Taq pol I Kwon Suk-Tae2009 Vmax 80 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Tgu Kwon Suk-Tae2009 Molecular Weight 9.026E+04 Dalton Technique: Sequence
Tgu Kwon Suk-Tae2009 3-5' Exonuclease (proofreading) Yes
Tgu Kwon Suk-Tae2009 Cloned or native Cloned in E. coli
Tgu Kwon Suk-Tae2009 Tagged No
Tgu Kwon Suk-Tae2009 Overall Error Rate 3.17E-06 errors/bp Technique: Blue/white screening
Tgu Kwon Suk-Tae2009 Full length or truncated Full length
Tgu Kwon Suk-Tae2009 Processivity 150bp
Tgu Kwon Suk-Tae2009 Vmax 30 /second Reaction: Nucleotide incorporation; Substrate: dNTPs

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