Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis

Kwon Suk-Tae, Shim Hyekyung, Choia Jeong Jin, Kima Yun Jae, Songa Jae-Geun, Kila Eui-Joon, Lee Jong Il
Enzyme and microbial technology (2009), Volume 45, Page 103

Polymerases:

Tgu,Pfu,Taq pol I

Topics:

Historical Protein Properties (MW, pI, ...), Kinetic Parameters, Fidelity, Nucleotide Incorporation, Exonuclease Activity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Pfu Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis Overall Error Rate 2.18E-06 errors/bp
Pfu Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis Vmax 21 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis Overall Error Rate 1.213E-05 errors/bp
Taq pol I Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis Vmax 80 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Tgu Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis Molecular Weight 9.026E+04 Dalton Technique: Sequence
Tgu Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis 3-5' Exonuclease (proofreading) Yes
Tgu Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis Cloned or native Cloned in E. coli
Tgu Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis Tagged No
Tgu Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis Overall Error Rate 3.17E-06 errors/bp Technique: Blue/white screening
Tgu Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis Full length or truncated Full length
Tgu Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis Processivity 150bp
Tgu Characterization and PCR optimization of the thermostable family B DNA polymerase from Thermococcus guaymasensis Vmax 30 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
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