Thermostable DNA polymerases.

Perler FB, Kumar S, Kong H
Adv Protein Chem (1996), Volume 48, Page 377
PubMed entry

Incomplete polymerases:

Polymerases:

Human Pol epsilon,Human Pol beta,Bst,Eco Pol II,Human Pol alpha,Vent,T7,Human Pol delta,Eco Pol I,Eco Pol III,Taq pol I,Human Pol gamma,Tma Pol III

Topics:

Historical Protein Properties (MW, pI, ...), Kinetic Parameters, Nucleotide Incorporation, Exonuclease Activity

Status:

new topics/pols set partial results complete validated

Results:

Polymerase Reference Property Result Context
Eco Pol I Thermostable DNA polymerases. Molecular Weight 1.03E+05 Dalton
Eco Pol I Thermostable DNA polymerases. 3-5' Exonuclease (proofreading) Yes
Eco Pol I Thermostable DNA polymerases. 5-3' Exonuclease Yes
Eco Pol I Thermostable DNA polymerases. Full length or truncated Full length
Eco Pol II Thermostable DNA polymerases. Molecular Weight 9E+04 Dalton
Eco Pol II Thermostable DNA polymerases. 3-5' Exonuclease (proofreading) Yes
Eco Pol II Thermostable DNA polymerases. 5-3' Exonuclease No
Eco Pol II Thermostable DNA polymerases. Full length or truncated Full length
Eco Pol III Thermostable DNA polymerases. Molecular Weight 1.3E+05 Dalton
Eco Pol III Thermostable DNA polymerases. 3-5' Exonuclease (proofreading) Yes
Eco Pol III Thermostable DNA polymerases. 5-3' Exonuclease No
Eco Pol III Thermostable DNA polymerases. Full length or truncated Full length
Human Pol alpha Thermostable DNA polymerases. 3-5' Exonuclease (proofreading) No
Human Pol alpha Thermostable DNA polymerases. 5-3' Exonuclease No
Human Pol alpha Thermostable DNA polymerases. Full length or truncated Full length
Human Pol beta Thermostable DNA polymerases. Molecular Weight 1.25E+05 Dalton
Human Pol beta Thermostable DNA polymerases. 3-5' Exonuclease (proofreading) No
Human Pol beta Thermostable DNA polymerases. 5-3' Exonuclease No
Human Pol beta Thermostable DNA polymerases. Full length or truncated Full length
Human Pol delta Thermostable DNA polymerases. Molecular Weight 1.25E+05 Dalton
Human Pol delta Thermostable DNA polymerases. 3-5' Exonuclease (proofreading) Yes
Human Pol delta Thermostable DNA polymerases. 5-3' Exonuclease No
Human Pol delta Thermostable DNA polymerases. Full length or truncated Full length
Human Pol epsilon Thermostable DNA polymerases. 3-5' Exonuclease (proofreading) Yes
Human Pol epsilon Thermostable DNA polymerases. 5-3' Exonuclease No
Human Pol epsilon Thermostable DNA polymerases. Full length or truncated Full length
Vent Thermostable DNA polymerases. Full length or truncated Full length
Vent Thermostable DNA polymerases. Maximum Product Length 1E+04bp
Vent Thermostable DNA polymerases. Processivity 7bp
T7 Thermostable DNA polymerases. 3-5' Exonuclease (proofreading) Yes
T7 Thermostable DNA polymerases. Full length or truncated Full length
T7 Thermostable DNA polymerases. Vmax 700 /second Reaction: 3-5' Exonuclease
T7 Thermostable DNA polymerases. Vmax 330 /second Reaction: Nucleotide incorporation; Substrate: dNTPs
Taq pol I Thermostable DNA polymerases. Molecular Weight 9.39E+04 Dalton Technique: Sequence
Taq pol I Thermostable DNA polymerases. 3-5' Exonuclease (proofreading) No
Taq pol I Thermostable DNA polymerases. 5-3' Exonuclease Yes
Human Pol gamma Thermostable DNA polymerases. 3-5' Exonuclease (proofreading) Yes
Human Pol gamma Thermostable DNA polymerases. 5-3' Exonuclease No
Human Pol gamma Thermostable DNA polymerases. Full length or truncated Full length
Bst Thermostable DNA polymerases. Molecular Weight 95kD
Bst Thermostable DNA polymerases. 3-5' Exonuclease (proofreading) No
Bst Thermostable DNA polymerases. 5-3' Exonuclease Yes
Tma Pol III Thermostable DNA polymerases. Molecular Weight 103kD
Tma Pol III Thermostable DNA polymerases. 3-5' Exonuclease (proofreading) Yes
Tma Pol III Thermostable DNA polymerases. 5-3' Exonuclease Yes
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