From E. coli (aliases:Klenow fragment pol domain)
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Welcome to the Polymerase Page

This page presents all the information in Polbase for KF(515-928).


At the top of the page you'll find a map of all positions of known mutants. Text links to mutants and digestion products are listed in the Mutants section.


If available, a representative structure is displayed at the upper right. All known structures are listed in the Structures section.

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Mutant of: Klenow fragment (324-514)

KF(515-928) is a family A enzyme from E. coli.

Engineered deletion of Klenow fragment, containing entire pol domain.  Designed based on KF structure.

Selected Properties for KF(515-928):

3'-5' exo activity 5'-3' exo activity Frameshift Rate Substitution Rate General Error Rate
No (1) no data 1.3e-05 errors/bp (1) 3.2e-05 errors/bp (1) no data
Year Authors Title Results by property
1990 Katarzyna Bebenek, Catherine M Joyce, M P Fitzgerald, Thomas A Kunkel The fidelity of DNA synthesis catalyz... 3-5' exo,Frameshift Error Rate,Nucleotide Substitution Rate,All properties
1986 P S Freemont, David L Ollis, Thomas A Steitz, Catherine M Joyce A domain of the Klenow fragment of Es...

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