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Polymerase: Sce Pol alpha

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Polymerase Reference Property Result Context
Sce Pol alpha Purification and characterization of the DNA polymerase alpha associated exonuclease: the RTH1 gene product. RNase H Yes
Sce Pol alpha Purification and characterization of the DNA polymerase alpha associated exonuclease: the RTH1 gene product. 5-3' Exonuclease Yes
Sce Pol alpha Purification and characterization of the 180- and 86-kilodalton subunits of the Saccharomyces cerevisiae DNA primase-DNA polymerase protein complex. The 180-kilodalton subunit has both DNA polymerase and 3'----5'-exonuclease activities. Molecular Weight 180kD
Sce Pol alpha Purification and characterization of the 180- and 86-kilodalton subunits of the Saccharomyces cerevisiae DNA primase-DNA polymerase protein complex. The 180-kilodalton subunit has both DNA polymerase and 3'----5'-exonuclease activities. 3-5' Exonuclease (proofreading) Yes
Sce Pol alpha Purification and characterization of the 180- and 86-kilodalton subunits of the Saccharomyces cerevisiae DNA primase-DNA polymerase protein complex. The 180-kilodalton subunit has both DNA polymerase and 3'----5'-exonuclease activities. Cloned or native Cloned in yeast
Sce Pol alpha Purification and characterization of the 180- and 86-kilodalton subunits of the Saccharomyces cerevisiae DNA primase-DNA polymerase protein complex. The 180-kilodalton subunit has both DNA polymerase and 3'----5'-exonuclease activities. Full length or truncated Full length
Sce Pol alpha Purification and characterization of the 180- and 86-kilodalton subunits of the Saccharomyces cerevisiae DNA primase-DNA polymerase protein complex. The 180-kilodalton subunit has both DNA polymerase and 3'----5'-exonuclease activities. Processivity 20bp
Sce Pol alpha Purification and characterization of the 180- and 86-kilodalton subunits of the Saccharomyces cerevisiae DNA primase-DNA polymerase protein complex. The 180-kilodalton subunit has both DNA polymerase and 3'----5'-exonuclease activities. KM 7uM Reaction: Nucleotide incorporation; Substrate: dATP; Experimental conditions: Mg++ (1mM)
Sce Pol alpha Distinct function of conserved amino acids in the fingers of Saccharomyces cerevisiae DNA polymerase alpha. Cloned or native Cloned in yeast
Sce Pol alpha Distinct function of conserved amino acids in the fingers of Saccharomyces cerevisiae DNA polymerase alpha. Tagged No
Sce Pol alpha Distinct function of conserved amino acids in the fingers of Saccharomyces cerevisiae DNA polymerase alpha. Nucleotide Substitution Rate 0.0034 errors/bp Technique: Forward mutational
Sce Pol alpha Distinct function of conserved amino acids in the fingers of Saccharomyces cerevisiae DNA polymerase alpha. Full length or truncated Full length
Sce Pol alpha Distinct function of conserved amino acids in the fingers of Saccharomyces cerevisiae DNA polymerase alpha. Processivity 12bp
Sce Pol alpha Distinct function of conserved amino acids in the fingers of Saccharomyces cerevisiae DNA polymerase alpha. Specific Activity 2.8E+04 units/mg Technique: Polymerase Assay (calf thymus DNA)
Sce Pol alpha Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme. Molecular Weight 180kD
Sce Pol alpha Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme. 3-5' Exonuclease (proofreading) No
Sce Pol alpha Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme. 5-3' Exonuclease No
Sce Pol alpha Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme. Processivity 10bp
Sce Pol alpha Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme. Specific Activity 90.2 units/mg Technique: Polymerase assay (poly[d(AT)]
Sce Pol alpha Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme. Specific Activity 219 units/mg Technique: Polymerase Assay (calf thymus DNA)
Sce Pol alpha Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme. KM 63nM Reaction: Polymerase-DNA interaction; Substrate: DNA template
Sce Pol alpha Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme. KM 3.8uM Reaction: Nucleotide incorporation; Substrate: dNTPs

Using Polbase tables:

Sorting:

Tables may be sorted by clicking on any of the column titles. A second click reverses the sort order. <Ctrl> + click on the column titles to sort by more than one column (e.g. family then name).

Filtering:

It is also possible to filter the table by typing into the search box above the table. This will instantly hide lines from the table that do not contain your search text.