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Polymerase: Sce Pol alpha

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Polymerase	Reference	Property	Result	Context
Sce Pol alpha	Purification and characterization of the DNA polymerase alpha associated exonuclease: the RTH1 gene product.	RNase H	Yes
Sce Pol alpha	Purification and characterization of the DNA polymerase alpha associated exonuclease: the RTH1 gene product.	5-3' Exonuclease	Yes
Sce Pol alpha	Purification and characterization of the 180- and 86-kilodalton subunits of the Saccharomyces cerevisiae DNA primase-DNA polymerase protein complex. The 180-kilodalton subunit has both DNA polymerase and 3'----5'-exonuclease activities.	Molecular Weight	180kD
Sce Pol alpha	Purification and characterization of the 180- and 86-kilodalton subunits of the Saccharomyces cerevisiae DNA primase-DNA polymerase protein complex. The 180-kilodalton subunit has both DNA polymerase and 3'----5'-exonuclease activities.	3-5' Exonuclease (proofreading)	Yes
Sce Pol alpha	Purification and characterization of the 180- and 86-kilodalton subunits of the Saccharomyces cerevisiae DNA primase-DNA polymerase protein complex. The 180-kilodalton subunit has both DNA polymerase and 3'----5'-exonuclease activities.	Cloned or native	Cloned in yeast
Sce Pol alpha	Purification and characterization of the 180- and 86-kilodalton subunits of the Saccharomyces cerevisiae DNA primase-DNA polymerase protein complex. The 180-kilodalton subunit has both DNA polymerase and 3'----5'-exonuclease activities.	Full length or truncated	Full length
Sce Pol alpha	Purification and characterization of the 180- and 86-kilodalton subunits of the Saccharomyces cerevisiae DNA primase-DNA polymerase protein complex. The 180-kilodalton subunit has both DNA polymerase and 3'----5'-exonuclease activities.	Processivity	20bp
Sce Pol alpha	Purification and characterization of the 180- and 86-kilodalton subunits of the Saccharomyces cerevisiae DNA primase-DNA polymerase protein complex. The 180-kilodalton subunit has both DNA polymerase and 3'----5'-exonuclease activities.	K_M	7uM	Reaction: Nucleotide incorporation; Substrate: dATP; Experimental conditions: Mg++ (1mM)
Sce Pol alpha	Distinct function of conserved amino acids in the fingers of Saccharomyces cerevisiae DNA polymerase alpha.	Cloned or native	Cloned in yeast
Sce Pol alpha	Distinct function of conserved amino acids in the fingers of Saccharomyces cerevisiae DNA polymerase alpha.	Tagged	No
Sce Pol alpha	Distinct function of conserved amino acids in the fingers of Saccharomyces cerevisiae DNA polymerase alpha.	Nucleotide Substitution Rate	0.0034 errors/bp	Technique: Forward mutational
Sce Pol alpha	Distinct function of conserved amino acids in the fingers of Saccharomyces cerevisiae DNA polymerase alpha.	Full length or truncated	Full length
Sce Pol alpha	Distinct function of conserved amino acids in the fingers of Saccharomyces cerevisiae DNA polymerase alpha.	Processivity	12bp
Sce Pol alpha	Distinct function of conserved amino acids in the fingers of Saccharomyces cerevisiae DNA polymerase alpha.	Specific Activity	2.8E+04 units/mg	Technique: Polymerase Assay (calf thymus DNA)
Sce Pol alpha	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Molecular Weight	180kD
Sce Pol alpha	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	3-5' Exonuclease (proofreading)	No
Sce Pol alpha	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	5-3' Exonuclease	No
Sce Pol alpha	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Processivity	10bp
Sce Pol alpha	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Specific Activity	90.2 units/mg	Technique: Polymerase assay (poly[d(AT)]
Sce Pol alpha	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	Specific Activity	219 units/mg	Technique: Polymerase Assay (calf thymus DNA)
Sce Pol alpha	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	K_M	63nM	Reaction: Polymerase-DNA interaction; Substrate: DNA template
Sce Pol alpha	Purification and characterization of DNA polymerase II from the yeast Saccharomyces cerevisiae. Identification of the catalytic core and a possible holoenzyme form of the enzyme.	K_M	3.8uM	Reaction: Nucleotide incorporation; Substrate: dNTPs